Key features and details
- Rabbit polyclonal to FYTTD1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FYTTD1 antibody
DescriptionRabbit polyclonal to FYTTD1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
Synthetic peptide, corresponding to a region within amino acids 1-50 of Human FYTTD1 (NP_115664.2).
- 293T, Jurkat, HeLa and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab125082 is affinity purified using an epitope specific to FYTTD1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab125082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 36 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionRequired for mRNA export from the nucleus to the cytoplasm. Acts as an adapter that uses the DDX39B/UAP56-NFX1 pathway to ensure efficient mRNA export and delivering to the nuclear pore. Associates with spliced and unspliced mRNAs simultaneously with THOC4.
Sequence similaritiesBelongs to the UIF family.
Cellular localizationNucleus > nucleoplasm. Nucleus speckle.
- Information by UniProt
- DKFZp761B1514 antibody
- Forty two three domain containing 1 antibody
- Forty two three domain containing protein 1 antibody
All lanes : Anti-FYTTD1 antibody (ab125082) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : HeLa cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 36 kDa
Exposure time: 30 seconds
Detection of FYTTD1 by Western Blot of Immunprecipitate.
ab125082 at 1µg/ml staining FYTTD1 in 293T whole cell lysate immunoprecipitated using ab125082 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab125082 has not yet been referenced specifically in any publications.