Anti-FYTTD1 antibody (ab125082)
Key features and details
- Rabbit polyclonal to FYTTD1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FYTTD1 antibody -
Description
Rabbit polyclonal to FYTTD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 1-50 of Human FYTTD1 (NP_115664.2).
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Positive control
- 293T, Jurkat, HeLa and NIH3T3 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab125082 is affinity purified using an epitope specific to FYTTD1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 36 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 36 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Required for mRNA export from the nucleus to the cytoplasm. Acts as an adapter that uses the DDX39B/UAP56-NFX1 pathway to ensure efficient mRNA export and delivering to the nuclear pore. Associates with spliced and unspliced mRNAs simultaneously with THOC4. -
Sequence similarities
Belongs to the UIF family. -
Cellular localization
Nucleus > nucleoplasm. Nucleus speckle. - Information by UniProt
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Database links
- Entrez Gene: 84248 Human
- Entrez Gene: 69823 Mouse
- Entrez Gene: 360726 Rat
- SwissProt: Q96QD9 Human
- SwissProt: Q91Z49 Mouse
- SwissProt: Q7TQ84 Rat
- Unigene: 277533 Human
- Unigene: 12831 Mouse
see all -
Alternative names
- DKFZp761B1514 antibody
- Forty two three domain containing 1 antibody
- Forty two three domain containing protein 1 antibody
see all
Images
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All lanes : Anti-FYTTD1 antibody (ab125082) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : HeLa cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 36 kDa
Exposure time: 30 seconds -
Detection of FYTTD1 by Western Blot of Immunprecipitate.
ab125082 at 1µg/ml staining FYTTD1 in 293T whole cell lysate immunoprecipitated using ab125082 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab125082 has not yet been referenced specifically in any publications.