Overview

  • Product name

  • Description

    Rabbit polyclonal to FZD10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human FZD10 aa 121-581.

  • Positive control

    • H1299, NIH 3T3, JC and BCL1 whole cell lysates.
  • General notes

     This product was previously labelled as Frizzled 10

     

Properties

Applications

Our Abpromise guarantee covers the use of ab137491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 65 kDa.

Target

  • Function

    Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • Tissue specificity

    Highest levels in the placenta and fetal kidney, followed by fetal lung and brain. In adult brain, abundantly expressed in the cerebellum, followed by cerebral cortex, medulla and spinal cord; very low levels in total brain, frontal lobe, temporal lobe and putamen. Weak expression detected in adult brain, heart, lung, skeletal muscle, pancreas, spleen and prostate.
  • Sequence similarities

    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • Domain

    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Post-translational
    modifications

    Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 350 antibody
    • CD 350 antigen antibody
    • CD350 antibody
    • Frizzled (Drosophila) homolog 10 antibody
    • Frizzled-10 antibody
    • FZ 10 antibody
    • Fz-10 antibody
    • FZ10 antibody
    • FZD 10 antibody
    • FZD10 antibody
    • FZD10_HUMAN antibody
    • FzE 7 antibody
    • FzE7 antibody
    • hFz 10 antibody
    • hFz10 antibody
    see all

Images

  • Anti-FZD10 antibody (ab137491) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 65 kDa



    7.5% SDS PAGE
  • All lanes : Anti-FZD10 antibody (ab137491) at 1/1000 dilution

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 65 kDa



    7.5% SDS PAGE

References

This product has been referenced in:

  • Chen X  et al. The hedgehog and Wnt/ß-catenin system machinery mediate myofibroblast differentiation of LR-MSCs in pulmonary fibrogenesis. Cell Death Dis 9:639 (2018). Read more (PubMed: 29844390) »
See 1 Publication for this product

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