Key features and details
- Rabbit polyclonal to FZD10
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FZD10 antibody
See all FZD10 primary antibodies
DescriptionRabbit polyclonal to FZD10
SpecificityBLAST analysis of the peptide immunogen showed no homology with other human proteins.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Horse, Hamster, Cow, Dog, Pig, Monkey, Common marmoset
Synthetic peptide corresponding to Human FZD10 (N terminal). Synthetic peptide, corresponding to 14 amino acids from the N-terminal extracellular domain of Human FZD10.
Database link: Q9ULW2
- Human fetal kidney tissue.
This product was previously labelled as Frizzled 10
Storage instructionsShipped at 4°C.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab150564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 6 - 16 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Tissue specificityHighest levels in the placenta and fetal kidney, followed by fetal lung and brain. In adult brain, abundantly expressed in the cerebellum, followed by cerebral cortex, medulla and spinal cord; very low levels in total brain, frontal lobe, temporal lobe and putamen. Weak expression detected in adult brain, heart, lung, skeletal muscle, pancreas, spleen and prostate.
Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain.
DomainLys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands.
modificationsUbiquitinated by ZNRF3, leading to its degradation by the proteasome.
Cellular localizationCell membrane.
- Information by UniProt
- CD 350 antibody
- CD 350 antigen antibody
- CD350 antibody
ab150564 has been referenced in 2 publications.
- Huo R et al. RNA Sequencing Reveals the Activation of Wnt Signaling in Low Flow Rate Brain Arteriovenous Malformations. J Am Heart Assoc 8:e012746 (2019). PubMed: 31170876
- Paradise CR et al. Molecular characterization of physis tissue by RNA sequencing. Gene 668:87-96 (2018). IHC-P ; Human . PubMed: 29775757