Anti-FZD10 antibody (ab150564)
Key features and details
- Rabbit polyclonal to FZD10
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FZD10 antibody
See all FZD10 primary antibodies -
Description
Rabbit polyclonal to FZD10 -
Host species
Rabbit -
Specificity
BLAST analysis of the peptide immunogen showed no homology with other human proteins. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Horse, Hamster, Cow, Dog, Pig, Monkey, Common marmoset -
Immunogen
Synthetic peptide corresponding to Human FZD10 (N terminal). Synthetic peptide, corresponding to 14 amino acids from the N-terminal extracellular domain of Human FZD10.
Database link: Q9ULW2 -
Positive control
- Human fetal kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 6 - 16 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 6 - 16 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. -
Tissue specificity
Highest levels in the placenta and fetal kidney, followed by fetal lung and brain. In adult brain, abundantly expressed in the cerebellum, followed by cerebral cortex, medulla and spinal cord; very low levels in total brain, frontal lobe, temporal lobe and putamen. Weak expression detected in adult brain, heart, lung, skeletal muscle, pancreas, spleen and prostate. -
Sequence similarities
Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain. -
Domain
Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands. -
Post-translational
modificationsUbiquitinated by ZNRF3, leading to its degradation by the proteasome. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 100398976 Common marmoset
- Entrez Gene: 515323 Cow
- Entrez Gene: 486232 Dog
- Entrez Gene: 11211 Human
- Entrez Gene: 93897 Mouse
- Omim: 606147 Human
- SwissProt: Q9ULW2 Human
- SwissProt: Q8BKG4 Mouse
see all -
Alternative names
- CD 350 antibody
- CD 350 antigen antibody
- CD350 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab150564 has been referenced in 2 publications.
- Huo R et al. RNA Sequencing Reveals the Activation of Wnt Signaling in Low Flow Rate Brain Arteriovenous Malformations. J Am Heart Assoc 8:e012746 (2019). PubMed: 31170876
- Paradise CR et al. Molecular characterization of physis tissue by RNA sequencing. Gene 668:87-96 (2018). IHC-P ; Human . PubMed: 29775757