Product nameAnti-FZD3 antibody
See all FZD3 primary antibodies
DescriptionRabbit polyclonal to FZD3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Macaque monkey
Synthetic peptide corresponding to Human FZD3 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human skeletal muscle tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab102965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 76 kDa).|
FunctionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Tissue specificityWidely expressed. Relatively high expression in the CNS, including regions of the limbic system, in kidney, pancreas, skeletal muscle, uterus and testis.
Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain.
DomainLys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands.
Cellular localizationMembrane. Cell membrane.
- Information by UniProt
- Frizzled 3 antibody
- Frizzled 3 seven transmembrane spanning receptor antibody
- Frizzled family receptor 3 antibody
Anti-FZD3 antibody (ab102965) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
The band observed at 74 kDa could potentially be a cleaved form of FZD3 due to the presence of a 22 amino acid signal peptide.
ab102965 has not yet been referenced specifically in any publications.