Overview

  • Product name

  • Description

    Rabbit polyclonal to FZD3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to Human FZD3 aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab113999)

  • Positive control

    • This antibody gave a positive signal in Human skeletal muscle tissue lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 76 kDa).

Target

  • Function

    Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • Tissue specificity

    Widely expressed. Relatively high expression in the CNS, including regions of the limbic system, in kidney, pancreas, skeletal muscle, uterus and testis.
  • Sequence similarities

    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • Domain

    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localization

    Membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Frizzled 3 antibody
    • Frizzled 3 seven transmembrane spanning receptor antibody
    • Frizzled family receptor 3 antibody
    • Frizzled homolog 3 (Drosophila) antibody
    • Frizzled homolog 3 antibody
    • Frizzled-3 antibody
    • Frizzled3 antibody
    • Fz 3 antibody
    • Fz-3 antibody
    • Fz3 antibody
    • FZD 3 antibody
    • Fzd3 antibody
    • FZD3_HUMAN antibody
    • hFz 3 antibody
    • hFz3 antibody
    see all

Images

  • Anti-FZD3 antibody (ab102965) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 76 kDa
    Observed band size: 74 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes


    The band observed at 74 kDa could potentially be a cleaved form of FZD3 due to the presence of a 22 amino acid signal peptide.

References

ab102965 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E9.5 mouse embryo section)
Specification
E9.5 mouse embryo section
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Paraformaldehyde

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Submitted Jan 28 2015

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