Key features and details
- Rabbit polyclonal to FZD3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab75233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 76 kDa.|
|ICC/IF||1/500 - 1/1000.|
FunctionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Tissue specificityWidely expressed. Relatively high expression in the CNS, including regions of the limbic system, in kidney, pancreas, skeletal muscle, uterus and testis.
Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain.
DomainLys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands.
Cellular localizationMembrane. Cell membrane.
- Information by UniProt
- Frizzled 3 antibody
- Frizzled 3 seven transmembrane spanning receptor antibody
- Frizzled family receptor 3 antibody
ab75233 at 1/500 dilution staining FZD3 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
All lanes : Anti-FZD3 antibody (ab75233) at 1/500 dilution
Lane 1 : HUVEC cell extracts
Lane 2 : HUVEC cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 76 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 117 kDa, 34 kDa. We are unsure as to the identity of these extra bands.
ab75233 has been referenced in 3 publications.
- Mo D et al. A tRNA fragment, 5'-tiRNAVal, suppresses the Wnt/ß-catenin signaling pathway by targeting FZD3 in breast cancer. Cancer Lett 457:60-73 (2019). PubMed: 31078732
- Gammons MV et al. Essential role of the Dishevelled DEP domain in a Wnt-dependent human-cell-based complementation assay. J Cell Sci 129:3892-3902 (2016). Human . PubMed: 27744318
- Wang SB et al. Temporal and spatial expression profiles of Frizzled 3 in the ovary during the estrous cycle. Genet Mol Res 15:N/A (2016). PubMed: 26909900