Key features and details
- Rabbit polyclonal to FZR1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FZR1 antibody
See all FZR1 primary antibodies
DescriptionRabbit polyclonal to FZR1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Human gastric cancer tissue; Mouse intestine and testis lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab217038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 55 kDa.|
|IHC-P||1/100 - 1/500.|
FunctionKey regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.
Tissue specificityIsoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the WD repeat CDC20/Fizzy family.
Contains 7 WD repeats.
modificationsPhosphorylated during mitosis, probably by maturation promoting factor (MPF), leading to its dissociation of the APC/C. Following DNA damage, it is dephosphorylated by CDC14B in G2 phase, leading to its reassociation with the APC/C, and allowing an efficient G2 DNA damage checkpoint.
Cellular localizationCytoplasm and Nucleus.
- Information by UniProt
- CDC20 like 1b antibody
- CDC20 like protein 1 antibody
- CDC20-like protein 1 antibody
Anti-FZR1 antibody (ab217038) at 1/300 dilution + Mouse testis lysate
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/500 dilution
Predicted band size: 55 kDa
Anti-FZR1 antibody (ab217038) at 1/300 dilution + Mouse intestine lysate
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 55 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human gastric cancer tissue labeling FZR1 with ab217038 at 1/200 dilution, followed by secondary antibody detection and DAB staining.
ab217038 has been referenced in 1 publication.
- Wang Y et al. P53 suppresses SENP3 phosphorylation to mediate G2 checkpoint. Cell Discov 6:21 (2020). PubMed: 32351703