Key features and details
- Mouse monoclonal [AR38.2] to FZR1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A/G purified
Our Abpromise guarantee covers the use of ab77885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2.5 µg/ml. Predicted molecular weight: 55 kDa.|
FunctionKey regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.
Tissue specificityIsoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the WD repeat CDC20/Fizzy family.
Contains 7 WD repeats.
modificationsPhosphorylated during mitosis, probably by maturation promoting factor (MPF), leading to its dissociation of the APC/C. Following DNA damage, it is dephosphorylated by CDC14B in G2 phase, leading to its reassociation with the APC/C, and allowing an efficient G2 DNA damage checkpoint.
Cellular localizationCytoplasm and Nucleus.
- Information by UniProt
- CDC20 like 1b antibody
- CDC20 like protein 1 antibody
- CDC20-like protein 1 antibody
ab77885 has been referenced in 1 publication.
- Holt JE et al. Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes. Development 137:1297-304 (2010). WB ; Mouse . PubMed: 20223764