Key features and details
- Mouse monoclonal [7E4F7] to G-CSF
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-G-CSF antibody [7E4F7]
See all G-CSF primary antibodies
DescriptionMouse monoclonal [7E4F7] to G-CSF
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human G-CSF aa 1-207. (Purified from E.coli).
MAGPATQSPMKLMALQLLLWHSALWTVQEATPLGPASSLPQSFLLKCLEQ VRKIQGDGAALQEKLVSECATYKLCHPEELVLLGHSLGIPWAPLSSCPSQ ALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTI WQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRV LRHLAQP
Database link: P09919
- Human G-CSF (aa1-207) recombinant protein; G-CSF (aa1-207)-hIgGFc transfected HEK293 cell lysate; Human esophageal cancer tissue; HeLa and A549 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab204989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 22 kDa.|
|ICC/IF||1/200 - 1/1000.|
|IHC-P||1/200 - 1/1000.|
FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Sequence similaritiesBelongs to the IL-6 superfamily.
modificationsO-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
- Information by UniProt
- C17orf33 antibody
- Colony stimulating factor 3 (granulocyte) antibody
- Colony stimulating factor 3 antibody
Anti-G-CSF antibody [7E4F7] (ab204989) at 1/500 dilution + Human G-CSF (aa1-207) recombinant protein
Predicted band size: 22 kDa
Expected MWt is 48.2 kDa.
All lanes : Anti-G-CSF antibody [7E4F7] (ab204989) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : G-CSF (aa1-207)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 22 kDa
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling G-CSF with ab204989 at 1/200 dilution with DAB staining.
Immunofluorescent analysis of A549 cells labeling G-CSF with ab204989 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescent analysis of HeLa cells labeling G-CSF with ab204989 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
ab204989 has not yet been referenced specifically in any publications.