Overview

  • Product name

    Anti-G-CSF antibody [8G5F7]
    See all G-CSF primary antibodies
  • Description

    Mouse monoclonal [8G5F7] to G-CSF
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human G-CSF aa 1-207. Expressed in E.coli.
    Sequence:

    MAGPATQSPM KLMALQLLLW HSALWTVQEA TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLVSECA TYKLCHPEEL VLLGHSLGIP WAPLSSCPSQ ALQLAGCLSQ LHSGLFLYQG LLQALEGISP ELGPTLDTLQ LDVADFATTI WQQMEELGMA PALQPTQGAM PAFASAFQRR AGGVLVASHL QSFLEVSYRV LRHLAQP


    Database link: P09919

  • Positive control

    • Human G-CSF (aa1-207) recombinant protein, G-CSF (aa1-207)-hIgGFc transfected HEK293 whole cell lysate, A549 or Hela cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    8G5F7
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab204998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 22 kDa.
Flow Cyt 1/200 - 1/400.
ICC/IF 1/200 - 1/1000.

Target

  • Function

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications

    O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • C17orf33 antibody
    • Colony stimulating factor 3 (granulocyte) antibody
    • Colony stimulating factor 3 antibody
    • CSF 3 antibody
    • CSF beta antibody
    • CSF3 antibody
    • CSF3_HUMAN antibody
    • CSF3OS antibody
    • Csfg antibody
    • Filgrastim antibody
    • G-CSF antibody
    • GCSA antibody
    • GCSF antibody
    • Granulocyte colony stimulating factor antibody
    • Granulocyte colony-stimulating factor antibody
    • Lenograstim antibody
    • Macrophage granulocyte inducer 2 antibody
    • MGC45931 antibody
    • MGI 2 antibody
    • Pluripoietin antibody
    see all

Images

  • All lanes : Anti-G-CSF antibody [8G5F7] (ab204998) at 1/500 dilution

    Lane 1 : HEK293 whole cell lysate.
    Lane 2 : Human G-CSF (aa1-207)-hIgGFc transfected HEK293 whole cell lysate.

    Predicted band size: 22 kDa

  • Flow cytometric analysis of A549 cells labelling G-CSF with ab204998 (green) at a 1/200 dilution and negative control (red).

  • Immunofluorescence analysis of HeLa cells labeling G-CSF with ab204998 at a 1/200 dilution (green), followed by Alexa Fluor® 555 phalloidin (red). Nuclear counterstain DRAQ5 fluorescent DNA dye used (blue).

  • Immunofluorescence analysis of A549 cells labeling G-CSF with ab204998 at a 1/200 dilution (green), followed by Alexa Fluor® 555 phalloidin (red). Nuclear counterstain DRAQ5 fluorescent DNA dye used (blue).

  • Anti-G-CSF antibody [8G5F7] (ab204998) at 1/500 dilution + Human G-CSF (aa1-207) recombinant protein

    Predicted band size: 22 kDa
    Additional bands at: 48.2 kDa. We are unsure as to the identity of these extra bands.

References

ab204998 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab204998.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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