Key features and details
- Rabbit polyclonal to G-CSF
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-G-CSF antibody
See all G-CSF primary antibodies
DescriptionRabbit polyclonal to G-CSF
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Pig
Recombinant fragment (His-tag) corresponding to Pig G-CSF aa 34-193. (Expressed in E.coli).
FLLKCLEQVRKIQADGAELQERLCATHKLCHPQELVLLGHSLGLPQASLS SCSSQALQLTGCLNQLHGGLVLYQGLLQALAGISPELAPALDILQLDVTD LATNIWLQMEDLRMAPASLPTQGTVPTFTSAFQRRAGGVLVVSQLQSFLE LAYRVLRYLA
Database link: O02837
- WB: Human leukocyte cell lysate; Mouse lymphocyte cell lysate: Mouse thymus tissue lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesFollowed by Protein A chromatography.
Our Abpromise guarantee covers the use of ab231277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 22 kDa.|
FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Sequence similaritiesBelongs to the IL-6 superfamily.
modificationsO-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
- Information by UniProt
- C17orf33 antibody
- Colony stimulating factor 3 (granulocyte) antibody
- Colony stimulating factor 3 antibody
All lanes : Anti-G-CSF antibody (ab231277) at 2 µg/ml
Lane 1 : Mouse lymphocyte cells
Lane 2 : Human leukocyte cells
Lane 3 : Mouse thymus tissue lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit Ab. at 1/2000 dilution
Predicted band size: 22 kDa
Anti-G-CSF antibody (ab231277) at 2 µg/ml + Recombinant pig G-SCF protein.
Predicted band size: 22 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231277 has not yet been referenced specifically in any publications.