Anti-G-CSF antibody (ab9691)
- Datasheet
- References (5)
- Protocols
Overview
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Product nameAnti-G-CSF antibody
See all G-CSF primary antibodies -
DescriptionRabbit polyclonal to G-CSF
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ELISA, Neutralising, Sandwich ELISA, IHC-P, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment corresponding to Human G-CSF aa 31-204. Produced in E.coli. Isoform Short of G-CSF.
Sequence:TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPW APLSSCPSQA LQLAGCLSQL HSGLFLYQGL LQALEGISPE LG PTLDTL QLDVADFATT IWQQMEELGM APALQPTQGA MPAFASAFQR RAGG VLVA SHLQSFLEVS YRVLRHLAQP
Database link: P09919-2 -
Positive control
- Purchase matching WB positive control:Recombinant human G-CSF protein
- Recombinant human G-CSF protein (ab9692) can be used as a positive control in WB.
Properties
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FormLyophilised:Reconstitute with 200µl of sterile water.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab9691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| ELISA | Use at an assay dependent concentration. To detect hG-CSF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hG-CSF. | |
| Neutralising | Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hG-CSF (0.5 ng/ml), a concentration of 0.04 - 0.06 µg/ml of this antibody is required. | |
| Sandwich ELISA | Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [5D7] to G-CSF (ab9818). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab9818 as Capture. |
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| IHC-P | Use a concentration of 0.25 µg/ml. | |
| Flow Cyt | 1/300. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
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Sequence similaritiesBelongs to the IL-6 superfamily.
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Post-translational
modificationsO-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells). -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 1440 Human
- Omim: 138970 Human
- SwissProt: P09919 Human
- Unigene: 2233 Human
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Alternative names
- C17orf33 antibody
- Colony stimulating factor 3 (granulocyte) antibody
- Colony stimulating factor 3 antibody
see all
Images
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Western blot - Anti-G-CSF antibody (ab9691) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-CSF antibody (ab9691)ab9691 staining G-CSF in human angiosarcoma section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen. -
Sandwich ELISA - Anti-G-CSF antibody (ab9691)Standard Curve for G-CSF (Analyte: ab51298) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [5D7] to G-CSF (ab9818) at 1ug/ml and Detector Antibody Rabbit polyclonal to G-CSF (ab9691) at 0.5ug/ml -
Western blot - Anti-G-CSF antibody (ab9691)
References
This product has been referenced in:
- Meyer MA et al. Breast and pancreatic cancer interrupt IRF8-dependent dendritic cell development to overcome immune surveillance. Nat Commun 9:1250 (2018). Read more (PubMed: 29593283) »
- Zhang L et al. G-CSF associates with neurogenesis and predicts prognosis and sensitivity to chemotherapy in pancreatic ductal adenocarcinoma. Cancer Manag Res 10:2767-2775 (2018). Read more (PubMed: 30147373) »
