Anti-G-CSF antibody (ab9691)
Key features and details
- Rabbit polyclonal to G-CSF
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-G-CSF antibody
See all G-CSF primary antibodies -
Description
Rabbit polyclonal to G-CSF -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment corresponding to Human G-CSF aa 1 to the C-terminus. Produced in E.coli. Isoform Short of G-CSF.
Database link: P09919-2 -
Positive control
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General notes
Although some customers have been successful in IHC we no longer batch test in this application. For an IHC validated antibody please see ab216607.
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use at an assay dependent concentration. To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
|
| Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml.
This antigen affinity purified antibody, in conjunction with ab271256 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hG-CSF |
| Notes |
|---|
|
WB
Use at an assay dependent concentration. To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. This antigen affinity purified antibody, in conjunction with ab271256 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hG-CSF |
Target
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Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsO-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells). -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 1440 Human
- Omim: 138970 Human
- SwissProt: P09919 Human
- Unigene: 2233 Human
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Alternative names
- C17orf33 antibody
- Colony stimulating factor 3 (granulocyte) antibody
- Colony stimulating factor 3 antibody
see all
Images
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Western blot - Anti-G-CSF antibody (ab9691)
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Sandwich ELISA - Anti-G-CSF antibody (ab9691)Standard Curve for G-CSF (Analyte: ab51298) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [5D7] to G-CSF (ab9818) at 1ug/ml and Detector Antibody Rabbit polyclonal to G-CSF (ab9691) at 0.5ug/ml
References (7)
ab9691 has been referenced in 7 publications.
- Andar A et al. Wood Microfluidics. Anal Chem 91:11004-11012 (2019). PubMed: 31361950
- Guo L et al. A high-risk luminal A dominant breast cancer subtype with increased mobility. Breast Cancer Res Treat N/A:N/A (2019). PubMed: 30778902
- Meyer MA et al. Breast and pancreatic cancer interrupt IRF8-dependent dendritic cell development to overcome immune surveillance. Nat Commun 9:1250 (2018). PubMed: 29593283
- Zhang L et al. G-CSF associates with neurogenesis and predicts prognosis and sensitivity to chemotherapy in pancreatic ductal adenocarcinoma. Cancer Manag Res 10:2767-2775 (2018). PubMed: 30147373
- Makino T et al. Diffuse fluorodeoxyglucose-positron uptake in the bone marrow of a patient with granulocyte colony-stimulating factor-producing pleomorphic carcinoma of the lung: A case report. Mol Clin Oncol 7:103-106 (2017). PubMed: 28685085
- Xue H et al. Tiaogeng Yijing decoction improves the pregnancy outcomes of patients with poor ovarian response undergoingin vitrofertilization-embryo transfer. Exp Ther Med 14:3935-3941 (2017). PubMed: 29043003
- Hollmén M et al. G-CSF regulates macrophage phenotype and associates with poor overall survival in human triple-negative breast cancer. Oncoimmunology 5:e1115177 (2016). IHC-P ; Human . PubMed: 27141367
