Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab9691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hG-CSF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hG-CSF.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hG-CSF (0.5 ng/ml), a concentration of 0.04 - 0.06 µg/ml of this antibody is required.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [5D7] to G-CSF (ab9818).

For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab9818 as Capture.
IHC-P Use a concentration of 0.25 µg/ml.
Flow Cyt 1/300.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
  • Sequence similarities
    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications
    O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • C17orf33 antibody
      • Colony stimulating factor 3 (granulocyte) antibody
      • Colony stimulating factor 3 antibody
      • CSF 3 antibody
      • CSF beta antibody
      • CSF3 antibody
      • CSF3_HUMAN antibody
      • CSF3OS antibody
      • Csfg antibody
      • Filgrastim antibody
      • G-CSF antibody
      • GCSA antibody
      • GCSF antibody
      • Granulocyte colony stimulating factor antibody
      • Granulocyte colony-stimulating factor antibody
      • Lenograstim antibody
      • Macrophage granulocyte inducer 2 antibody
      • MGC45931 antibody
      • MGI 2 antibody
      • Pluripoietin antibody
      see all

    Images

    • Western blot - Anti-G-CSF antibody (ab9691)
      Western blot - Anti-G-CSF antibody (ab9691)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-CSF antibody (ab9691)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-CSF antibody (ab9691)
      ab9691 staining G-CSF in human angiosarcoma section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.
    • Sandwich ELISA - Anti-G-CSF antibody (ab9691)
      Sandwich ELISA - Anti-G-CSF antibody (ab9691)
      Standard Curve for G-CSF (Analyte: ab51298) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [5D7] to G-CSF (ab9818) at 1ug/ml and Detector Antibody Rabbit polyclonal to G-CSF (ab9691) at 0.5ug/ml
    • Western blot - Anti-G-CSF antibody (ab9691)
      Western blot - Anti-G-CSF antibody (ab9691)

    References

    This product has been referenced in:
    • Meyer MA  et al. Breast and pancreatic cancer interrupt IRF8-dependent dendritic cell development to overcome immune surveillance. Nat Commun 9:1250 (2018). Read more (PubMed: 29593283) »
    • Makino T  et al. Diffuse fluorodeoxyglucose-positron uptake in the bone marrow of a patient with granulocyte colony-stimulating factor-producing pleomorphic carcinoma of the lung: A case report. Mol Clin Oncol 7:103-106 (2017). Read more (PubMed: 28685085) »

    See all 4 Publications for this product

    Publishing research using ab9691? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    14 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-G-CSF antibody

     Good
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    Lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More
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    Abcam user community

    Verified customer

    Submitted May 19 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-G-CSF antibody

     Good
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
    Permeabilization
    No
    Specification
    Lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted May 19 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-G-CSF antibody

     Good
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    Heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% Formalin
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted May 19 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-G-CSF antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    Heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% Normal buffered formalin
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted May 19 2017

    Western blot abreview for Anti-G-CSF antibody

     Inconclusive
    Abreviews
    Application
    Western blot
    Loading amount
    60 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Rat Cell lysate - whole cell (PC-12 cells)
    Specification
    PC-12 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
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    Abcam user community

    Verified customer

    Submitted Jun 17 2013

    Hi can you please specify for our records what would be the expiry date after reconstitution of the lyophilized material as suggested by you.

    The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8oC. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

    For product ab9691 (anti-G-CSF antibody), are there recommended expiration/stability dates for the lyophilized and the reconstituted product, stored at -20°C?



    There is no real expiry date on all our antibodies. Usually the life is 10 years if stored in -20. Minimal freeze thaw cycles definitely extend the life of the antibody.

    Dear order team,

    Our customer would like to purchaseab9691 with Lot#GR7283-8.
    Please let me know if you havesame lot#in stock.
    Thank you in advance.

    Best regards,

    Thank you for contacting us. We do not currently have that particular lot in stock but we do have lots of antibody produced from the same bleed and purification. The only difference would be that the antibody was aliquotted by us at a different time. T...

    Read More

    How long can this antibody be stored at -20C?

    Thank you for your call today and for your question.

    We don't assign specific expiration dates for our products (our guarantee is for 6 months after purchase), but we expect an antibody stored at -20C to be viable for up to 5 years.

    Read More

    Dear Technical, Good day! We have a customer who has purchased the above antibody and would like a certificate of analysis for this.

    Thank you for contacting. Please find attached the Certificate of Analysis for ab9691. If there is anything else I can help you with, please let me know.

    1-10 of 14 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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