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    g-csf-antibody-ab9691.pdf

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Immunology Innate Immunity Cytokines CSFs
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Anti-G-CSF antibody (ab9691)

  • Datasheet
  • SDS
Reviews (6)Q&A (8)References (7)

Product price, shipping and contact information

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Abpromise

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Find out more.

Western blot - Anti-G-CSF antibody (ab9691)
  • Sandwich ELISA - Anti-G-CSF antibody (ab9691)

Key features and details

  • Rabbit polyclonal to G-CSF
  • Suitable for: WB, Sandwich ELISA
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG

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Overview

  • Product name

    Anti-G-CSF antibody
    See all G-CSF primary antibodies
  • Description

    Rabbit polyclonal to G-CSF
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human G-CSF aa 1 to the C-terminus. Produced in E.coli. Isoform Short of G-CSF.
    Database link: P09919-2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Purchase matching WB positive control:Recombinant human G-CSF protein
  • General notes

    Although some customers have been successful in IHC we no longer batch test in this application. For an IHC validated antibody please see ab216607.

Properties

  • Form

    Lyophilized:Reconstitute with 200µl of sterile water.
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    No preservative, sterile filtered
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Light chain type

    unknown
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • CSFs
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant human G-CSF protein (ab9692)
  • Recombinant Protein

    • Recombinant human G-CSF protein (ab167700)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB (2)
Use at an assay dependent concentration. To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml.

This antigen affinity purified antibody, in conjunction with ab271256 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hG-CSF

Notes
WB
Use at an assay dependent concentration. To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml.

This antigen affinity purified antibody, in conjunction with ab271256 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hG-CSF

Target

  • Function

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications

    O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P09919 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1440 Human
    • Omim: 138970 Human
    • SwissProt: P09919 Human
    • Unigene: 2233 Human
    • Alternative names

      • C17orf33 antibody
      • Colony stimulating factor 3 (granulocyte) antibody
      • Colony stimulating factor 3 antibody
      • CSF 3 antibody
      • CSF beta antibody
      • CSF3 antibody
      • CSF3_HUMAN antibody
      • CSF3OS antibody
      • Csfg antibody
      • Filgrastim antibody
      • G-CSF antibody
      • GCSA antibody
      • GCSF antibody
      • Granulocyte colony stimulating factor antibody
      • Granulocyte colony-stimulating factor antibody
      • Lenograstim antibody
      • Macrophage granulocyte inducer 2 antibody
      • MGC45931 antibody
      • MGI 2 antibody
      • Pluripoietin antibody
      see all

    Images

    • Western blot - Anti-G-CSF antibody (ab9691)
      Western blot - Anti-G-CSF antibody (ab9691)
      Anti-G-CSF antibody (ab9691) at 1 µg/ml + Recombinant human G-CSF protein (ab73371) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Exposure time: 2 minutes
    • Sandwich ELISA - Anti-G-CSF antibody (ab9691)
      Sandwich ELISA - Anti-G-CSF antibody (ab9691)
      Standard Curve for G-CSF (Analyte: ab51298) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [5D7] to G-CSF (ab9818) at 1ug/ml and Detector Antibody Rabbit polyclonal to G-CSF (ab9691) at 0.5ug/ml

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (7)

    Publishing research using ab9691? Please let us know so that we can cite the reference in this datasheet.

    ab9691 has been referenced in 7 publications.

    • Andar A  et al. Wood Microfluidics. Anal Chem 91:11004-11012 (2019). PubMed: 31361950
    • Guo L  et al. A high-risk luminal A dominant breast cancer subtype with increased mobility. Breast Cancer Res Treat N/A:N/A (2019). PubMed: 30778902
    • Meyer MA  et al. Breast and pancreatic cancer interrupt IRF8-dependent dendritic cell development to overcome immune surveillance. Nat Commun 9:1250 (2018). PubMed: 29593283
    • Zhang L  et al. G-CSF associates with neurogenesis and predicts prognosis and sensitivity to chemotherapy in pancreatic ductal adenocarcinoma. Cancer Manag Res 10:2767-2775 (2018). PubMed: 30147373
    • Makino T  et al. Diffuse fluorodeoxyglucose-positron uptake in the bone marrow of a patient with granulocyte colony-stimulating factor-producing pleomorphic carcinoma of the lung: A case report. Mol Clin Oncol 7:103-106 (2017). PubMed: 28685085
    • Xue H  et al. Tiaogeng Yijing decoction improves the pregnancy outcomes of patients with poor ovarian response undergoingin vitrofertilization-embryo transfer. Exp Ther Med 14:3935-3941 (2017). PubMed: 29043003
    • Hollmén M  et al. G-CSF regulates macrophage phenotype and associates with poor overall survival in human triple-negative breast cancer. Oncoimmunology 5:e1115177 (2016). IHC-P ; Human . PubMed: 27141367

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 14 Abreviews or Q&A

    Question

    Hi can you please specify for our records what would be the expiry date after reconstitution of the lyophilized material as suggested by you.

    Read More

    Abcam community

    Verified customer

    Asked on May 03 2013

    Answer

    The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8oC. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

    Read More

    Abcam Scientific Support

    Answered on May 03 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-G-CSF antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    Lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted May 19 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-G-CSF antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
    Permeabilization
    No
    Specification
    Lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted May 19 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-G-CSF antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    Heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% Formalin
    Read More

    Abcam user community

    Verified customer

    Submitted May 19 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-G-CSF antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    Heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% Normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted May 19 2017

    Western blot abreview for Anti-G-CSF antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Western blot
    Loading amount
    60 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Rat Cell lysate - whole cell (PC-12 cells)
    Specification
    PC-12 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 17 2013

    Question

    For product ab9691 (anti-G-CSF antibody), are there recommended expiration/stability dates for the lyophilized and the reconstituted product, stored at -20°C?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 14 2013

    Answer



    There is no real expiry date on all our antibodies. Usually the life is 10 years if stored in -20. Minimal freeze thaw cycles definitely extend the life of the antibody.

    Read More

    Abcam Scientific Support

    Answered on Feb 14 2013

    Question

    Dear order team,

    Our customer would like to purchaseab9691 with Lot#GR7283-8.
    Please let me know if you havesame lot#in stock.
    Thank you in advance.

    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 30 2012

    Answer

    Thank you for contacting us. We do not currently have that particular lot in stock but we do have lots of antibody produced from the same bleed and purification. The only difference would be that the antibody was aliquotted by us at a different time. The lots available are GR7283-9 and GR7283-10. Please specify when placing the order that you would like one of these.

    I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

    Read More

    Abcam Scientific Support

    Answered on Aug 30 2012

    Question

    How long can this antibody be stored at -20C?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 05 2012

    Answer

    Thank you for your call today and for your question.

    We don't assign specific expiration dates for our products (our guarantee is for 6 months after purchase), but we expect an antibody stored at -20C to be viable for up to 5 years.

    I hope this information will be useful, but please let me know if you have further questions or if there is anything else that we can do for you. Have a nice day!

    Read More

    Abcam Scientific Support

    Answered on Jul 05 2012

    Question

    Dear Technical, Good day! We have a customer who has purchased the above antibody and would like a certificate of analysis for this.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 22 2011

    Answer

    Thank you for contacting. Please find attached the Certificate of Analysis for ab9691. If there is anything else I can help you with, please let me know.

    Read More

    Abcam Scientific Support

    Answered on Dec 22 2011

    1-10 of 14 Abreviews or Q&A

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