Anti-G-CSF antibody (ab9691)

Rabbit polyclonal G-CSF antibody. Validated in WB, ELISA, IHC, Neut, Flow Cyt, sELISA and tested in Human. Cited in 5 publication(s). Independently reviewed in 6 review(s).

Overview

  • Product name
  • Description
    Rabbit polyclonal to G-CSF
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, Neutralising, Sandwich ELISA, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human G-CSF aa 31-204. Produced in E.coli. Isoform Short of G-CSF.
    Sequence:

    TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPW APLSSCPSQA LQLAGCLSQL HSGLFLYQGL LQALEGISPE LG PTLDTL QLDVADFATT IWQQMEELGM APALQPTQGA MPAFASAFQR RAGG VLVA SHLQSFLEVS YRVLRHLAQP


    Database link: P09919-2

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab9691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hG-CSF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hG-CSF.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hG-CSF (0.5 ng/ml), a concentration of 0.04 - 0.06 µg/ml of this antibody is required.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [5D7] to G-CSF (ab9818).

For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab9818 as Capture.

IHC-P Use a concentration of 0.25 µg/ml.
Flow Cyt 1/300.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
  • Sequence similarities
    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications
    O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C17orf33 antibody
    • Colony stimulating factor 3 (granulocyte) antibody
    • Colony stimulating factor 3 antibody
    • CSF 3 antibody
    • CSF beta antibody
    • CSF3 antibody
    • CSF3_HUMAN antibody
    • CSF3OS antibody
    • Csfg antibody
    • Filgrastim antibody
    • G-CSF antibody
    • GCSA antibody
    • GCSF antibody
    • Granulocyte colony stimulating factor antibody
    • Granulocyte colony-stimulating factor antibody
    • Lenograstim antibody
    • Macrophage granulocyte inducer 2 antibody
    • MGC45931 antibody
    • MGI 2 antibody
    • Pluripoietin antibody
    see all

Images

  • ab9691 staining G-CSF in human angiosarcoma section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.
  • Standard Curve for G-CSF (Analyte: ab51298) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [5D7] to G-CSF (ab9818) at 1ug/ml and Detector Antibody Rabbit polyclonal to G-CSF (ab9691) at 0.5ug/ml

References

This product has been referenced in:
  • Meyer MA  et al. Breast and pancreatic cancer interrupt IRF8-dependent dendritic cell development to overcome immune surveillance. Nat Commun 9:1250 (2018). Read more (PubMed: 29593283) »
  • Zhang L  et al. G-CSF associates with neurogenesis and predicts prognosis and sensitivity to chemotherapy in pancreatic ductal adenocarcinoma. Cancer Manag Res 10:2767-2775 (2018). Read more (PubMed: 30147373) »
See all 5 Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

Submitted May 19 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

Submitted May 19 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Formalin

Abcam user community

Verified customer

Submitted May 19 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Normal buffered formalin

Abcam user community

Verified customer

Submitted May 19 2017

Abreviews
Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Rat Cell lysate - whole cell (PC-12 cells)
Specification
PC-12 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jun 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Purified protein (E.coli)
Loading amount
3 µg
Specification
E.coli
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Chul sOO Cheong

Verified customer

Submitted Feb 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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