Anti-G-CSF antibody [CSF3/900] - BSA and Azide free (ab212315)

Overview

  • Product name

    Anti-G-CSF antibody [CSF3/900] - BSA and Azide free
    See all G-CSF primary antibodies
  • Description

    Mouse monoclonal [CSF3/900] to G-CSF - BSA and Azide free
  • Host species

    Mouse
  • Specificity

    This antibody recognizes G-CSF in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Macaque monkey
  • Immunogen

    Recombinant full length protein.
    Database link: P09919

  • Positive control

    • HL60 cells; Tonsil or lymph node tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab212315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5-1µg for 106 cells.

in 0.1ml volume

ICC/IF Use a concentration of 1 - 2 µg/ml.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

for 30 minutes at RT

Target

  • Function

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications

    O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • C17orf33 antibody
    • Colony stimulating factor 3 (granulocyte) antibody
    • Colony stimulating factor 3 antibody
    • CSF 3 antibody
    • CSF beta antibody
    • CSF3 antibody
    • CSF3_HUMAN antibody
    • CSF3OS antibody
    • Csfg antibody
    • Filgrastim antibody
    • G-CSF antibody
    • GCSA antibody
    • GCSF antibody
    • Granulocyte colony stimulating factor antibody
    • Granulocyte colony-stimulating factor antibody
    • Lenograstim antibody
    • Macrophage granulocyte inducer 2 antibody
    • MGC45931 antibody
    • MGI 2 antibody
    • Pluripoietin antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling G-CSF with ab212315 at 1µg/ml.

References

ab212315 has not yet been referenced specifically in any publications.

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