Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)
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Overview
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Product nameAnti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free
See all G-CSF primary antibodies -
DescriptionRabbit monoclonal [EPR3203(N)(B)] to G-CSF - BSA and Azide free
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Host speciesRabbit
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Tested applicationsSuitable for: IP, ICC/IF, Flow Cyt, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human G-CSF aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: P09919 -
Positive control
- WB: K562, mouse brain, rat brain, KM3, NCI-H460 and HT-1376 cell lysates. ICC/IF: BxPC-3 and HT-1376 cells. IP: G-CSF IP in K562 cell lysate. Flow Cyt: K562 cells.
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General notes
The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).
ab236157 is a PBS-only buffer format of ab181053. Please refer to ab181053 for recommended dilutions, protocols, and image data.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferConstituent: PBS
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Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR3203(N)(B)
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab236157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
Target
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FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
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Sequence similaritiesBelongs to the IL-6 superfamily.
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Post-translational
modificationsO-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells). -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 1440 Human
- Entrez Gene: 12985 Mouse
- Entrez Gene: 25610 Rat
- Omim: 138970 Human
- SwissProt: P09919 Human
- SwissProt: P09920 Mouse
- Unigene: 2233 Human
- Unigene: 1238 Mouse
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Alternative names
- C17orf33 antibody
- Colony stimulating factor 3 (granulocyte) antibody
- Colony stimulating factor 3 antibody
see all
Images
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![Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)](https://www.abcam.com/ps/products/236/ab236157/Images/ab236157-6-ab181053290781ab181053IP1.jpg)
Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)ab181053 (purified) at 1:100 dilution (2µg) immunoprecipitating G-CSF in K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate.
Lane 1 (input): K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
Lane 2 (+): ab181053 and K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181053 in K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate.
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181053).
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![Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)](https://www.abcam.com/ps/products/236/ab236157/Images/ab236157-5-ab181053290765ab181053ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)Immunocytochemistry/ Immunofluorescence analysis of BxPC-3 (Human pancreas adenocarcinoma epithelial cell) cells labeling G-CSF with Purified ab181053 at 1:500 dilution (4.0μg/ml). Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear was used as a counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181053).
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![Flow Cytometry - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)](https://www.abcam.com/ps/products/236/ab236157/Images/ab236157-4-ab181053272330ab181053FC1.jpg)
Flow Cytometry - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)Flow Cytometry analysis of K562 (human chronic myelogenous leukemia) cells labeling G-CSF with purified ab181053 at 1/200 dilution (10µg/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181053).
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![Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)](https://www.abcam.com/ps/products/236/ab236157/Images/ab236157-3-ab181053213071ab181053c.jpg)
Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)Western blot analysis of immunoprecipitation pellet from K562 cell lysate (lane 1) or a Negative control (lane 2) immunoprecipitated using unpurified ab181053 at 1/20 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181053).
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![Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)](https://www.abcam.com/ps/products/236/ab236157/Images/ab236157-2-ab181053213070ab181053b.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)Immunofluorescent analysis of HT-1376 cells (paraformaldehyde-fixed, 4%) labeling G-CSF with unpurified ab181053 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181053).
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![Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)](https://www.abcam.com/ps/products/236/ab236157/Images/ab236157-311947-053.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)Immunocytochemistry/ Immunofluorescence analysis of BxPC-3 (Human pancreas adenocarcinoma epithelial cell) cells labeling G-CSF with purified ab181053 at 1:500 dilution (4.0μg/ml). Cells were fixed in 100% methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear was used as a counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181053).
Datasheets and documents
References
ab236157 has not yet been referenced specifically in any publications.
