Recombinant Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3203(N)(B)] to G-CSF - BSA and Azide free
- Suitable for: IP, ICC/IF, Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free
See all G-CSF primary antibodies -
Description
Rabbit monoclonal [EPR3203(N)(B)] to G-CSF - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A549, K-562, HepG2, HeLa, MCF7, MOLT-4, PC-3, KM3, NCI-H460 and HT-1376 cell lysates; Mouse and rat brain lysates. ICC/IF: BxPC-3 and HT-1376 cells. IP: G-CSF IP in K562 cell lysate. Flow Cyt: K562 cells.
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General notes
Ab236157 is the carrier-free version of ab181053. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab236157 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3203(N)(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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Notes |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
Target
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Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsO-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells). -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 1440 Human
- Entrez Gene: 12985 Mouse
- Entrez Gene: 25610 Rat
- Omim: 138970 Human
- SwissProt: P09919 Human
- SwissProt: P09920 Mouse
- Unigene: 2233 Human
- Unigene: 1238 Mouse
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Alternative names
- C17orf33 antibody
- Colony stimulating factor 3 (granulocyte) antibody
- Colony stimulating factor 3 antibody
see all
Images
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All lanes : Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053) at 1/1000 dilution
Lane 1 : A549 (Human lung carcinoma epithelial cell) cell lysate
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate
Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) cell lysate
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate
Lane 5 : MCF7 (Human breast adenocarcinoma epithelial cell) cell lysate
Lane 6 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cell lysate
Lane 7 : PC-3 (Human prostate adenocarcinoma epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 4 s
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Flow Cytometry analysis of K562 (human chronic myelogenous leukemia) cells labeling G-CSF with purified ab181053 at 1/200 dilution (10µg/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181053).
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Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)
Immunocytochemistry/ Immunofluorescence analysis of BxPC-3 (Human pancreas adenocarcinoma epithelial cell) cells labeling G-CSF with Purified ab181053 at 1:500 dilution (4.0μg/ml). Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear was used as a counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181053).
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ab181053 (purified) at 1:100 dilution (2µg) immunoprecipitating G-CSF in K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate.
Lane 1 (input): K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
Lane 2 (+): ab181053 and K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181053 in K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate.
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181053).
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Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)
Immunofluorescent analysis of HT-1376 cells (paraformaldehyde-fixed, 4%) labeling G-CSF with unpurified ab181053 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181053).
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Western blot analysis of immunoprecipitation pellet from K562 cell lysate (lane 1) or a Negative control (lane 2) immunoprecipitated using unpurified ab181053 at 1/20 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181053).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab236157 has not yet been referenced specifically in any publications.