Anti-G gamma12 antibody (ab204757)
Key features and details
- Rabbit polyclonal to G gamma12
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-G gamma12 antibody
See all G gamma12 primary antibodies -
Description
Rabbit polyclonal to G gamma12 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan -
Immunogen
Recombinant fragment corresponding to Human G gamma12 aa 1 to the C-terminus.
Database link: Q9UBI6 -
Positive control
- IHC-P: Human small intestine tissue; ICC: U-2 OS whole cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
|
IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
G gamma12 has signal transducer activity in G-protein coupled receptor protein signaling pathway. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 286850 Cow
- Entrez Gene: 55970 Human
- Entrez Gene: 14701 Mouse
- Entrez Gene: 100173039 Orangutan
- Entrez Gene: 114120 Rat
- SwissProt: Q28024 Cow
- SwissProt: Q9UBI6 Human
- SwissProt: Q544P3 Mouse
see all -
Alternative names
- G-protein gamma-12 subunit antibody
- GNG12 antibody
- Guanine nucleotide binding protein (G protein) gamma 12 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human small intestine tissue labeling G gamma12 (high expression) with ab204757 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling G gamma12 in the mitochondria with ab204757 at 2 µg/ml.
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Immunohistochemical analysis of paraffin-embedded human liver tissue labeling G gamma12 (low expression) with ab204757 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab204757 has not yet been referenced specifically in any publications.