Anti-G protein alpha Inhibitor 2 antibody (ab20392)


  • Product name
    Anti-G protein alpha Inhibitor 2 antibody
    See all G protein alpha Inhibitor 2 primary antibodies
  • Description
    Rabbit polyclonal to G protein alpha Inhibitor 2
  • Host species
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Other Immunogen Type corresponding to Human G protein alpha Inhibitor 2.



Our Abpromise guarantee covers the use of ab20392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 40 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.


  • Function
    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.
  • Sequence similarities
    Belongs to the G-alpha family. G(i/o/t/z) subfamily.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cell membrane. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenylate cyclase inhibiting G alpha protein antibody
    • Adenylate cyclase-inhibiting G alpha protein antibody
    • GIP antibody
    • GNAI 2 antibody
    • Gnai2 antibody
    • GNAI2_HUMAN antibody
    • GNAI2B antibody
    • GTP binding regulatory protein Gi alpha 2 chain antibody
    • Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 2 antibody
    • Guanine nucleotide-binding protein G(i) subunit alpha-2 antibody
    • H LUCA15.1 antibody
    • H LUCA16.1 antibody
    • WUGSC:H LUCA15.1 antibody
    • WUGSC:H LUCA16.1 antibody
    see all


  • Lanes 1 & 3 : Anti-G protein alpha Inhibitor 2 antibody (ab20392) at 1/1000 dilution
    Lane 2 : No primary

    All lanes : Rat RH7777 whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Lane 1 : No secondary
    Lanes 2-3 : Alkaline phosphatase conjugated goat anti-rabbit

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

    Exposure time: 10 minutes

    See Abreview


This product has been referenced in:
  • Ramírez VT  et al. Wnt-5a/Frizzled9 Receptor Signaling through the Gao-Gß? Complex Regulates Dendritic Spine Formation. J Biol Chem 291:19092-107 (2016). Read more (PubMed: 27402827) »
  • Fu X  et al. MicroRNA-222-3p/GNAI2/AKT axis inhibits epithelial ovarian cancer cell growth and associates with good overall survival. Oncotarget 7:80633-80654 (2016). WB ; Human . Read more (PubMed: 27811362) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for sending the image again.

As my colleague mentioned you may need to increase the denaturing and reducing conditions with this specific cell line.

My suggestion is to lysate the cells with RIPA buffer, a recipe for this ...

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Thank you for your inquiry.

I am sorry to hear that you are getting results that are hard to interpret.

I believe the 120kDa band is not a complex containing G protein alpha Inhibitor 2 since you are using denaturing and reducing co...

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Thank you for contacting us.

The information I was able to obtain regarding the immunogen is as follows. Any further details are considered proprietary.

The immunogen for this antibody is a synthetic peptide surrounding amino acid ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Rat Cell lysate - whole cell (RH7777 Cells)
Loading amount
15 µg
RH7777 Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%

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Verified customer

Submitted Jul 31 2006


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