Overview

  • Product name
    Anti-G protein alpha Inhibitor 2 antibody [EPR9469]
    See all G protein alpha Inhibitor 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR9469] to G protein alpha Inhibitor 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human G protein alpha Inhibitor 2. The exact sequence is proprietary.
    Database link: P04899

  • Positive control
    • Recombinant Human G protein alpha Inhibitor 2 (ab134531) can be used as a positive control in WB. U937 and U-87 MG cell lysates; fetal brain lysate; kidney and pancreas tissues; U937 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 40 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.
    • Sequence similarities
      Belongs to the G-alpha family. G(i/o/t/z) subfamily.
    • Cellular localization
      Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cell membrane. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adenylate cyclase inhibiting G alpha protein antibody
      • Adenylate cyclase-inhibiting G alpha protein antibody
      • GIP antibody
      • GNAI 2 antibody
      • Gnai2 antibody
      • GNAI2_HUMAN antibody
      • GNAI2B antibody
      • GTP binding regulatory protein Gi alpha 2 chain antibody
      • Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 2 antibody
      • Guanine nucleotide-binding protein G(i) subunit alpha-2 antibody
      • H LUCA15.1 antibody
      • H LUCA16.1 antibody
      • WUGSC:H LUCA15.1 antibody
      • WUGSC:H LUCA16.1 antibody
      see all

    Images

    • ab157204 showing +ve staining in Human normal liver.
    • ab157204 showing +ve staining in Human normal brain.
    • All lanes : Anti-G protein alpha Inhibitor 2 antibody [EPR9469] (ab157204) at 1/10000 dilution

      Lane 1 : fetal brain lysate
      Lane 2 : U937 cell lysate
      Lane 3 : U-87 MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 40 kDa

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling G protein alpha Inhibitor 2 using ab157204 at a 1/50 dilution.
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling G protein alpha Inhibitor 2 using ab157204 at a 1/50 dilution.
    • Flow cytometry analysis of permeabilized U937 cells labeling G protein alpha Inhibitor 2 (red), using ab157204 at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)

    References

    This product has been referenced in:
    • Bissinger R  et al. Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gai2. Sci Rep 6:30925 (2016). WB . Read more (PubMed: 27499046) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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