Anti-G protein alpha S antibody [2A2B7] (ab204996)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G protein alpha S antibody [2A2B7] (ab204996)](https://www.abcam.com/ps/products/204/ab204996/Images/ab204996-247896-anti-g-protein-alpha-antibody-2a2b7-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G protein alpha S antibody [2A2B7] (ab204996)")
Key features and details
- Mouse monoclonal [2A2B7] to G protein alpha S
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-G protein alpha S antibody [2A2B7]
See all GNAS primary antibodies -
Description
Mouse monoclonal [2A2B7] to G protein alpha S -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human G protein alpha aa 42-188. Expressed in E.coli.
Sequence:RLLLLGAGE SGKSTIVKQM RILHVNGFNG EGGEEDPQAA RSNSDGEKAT KVQDIKNNLK EAIETIVAAM SNLVPPVELA NPENQFRVDY ILSVMNVPDF DFPPEFYEHA KALWEDEGVR ACYERSNEYQ LIDCAQYFLD KIDVIKQA
Database link: P63092 -
Positive control
- Human G protein alpha (aa42-188) recombinant protein, G protein alpha (aa42-188)-hIgGFc transfected HEK293 whole cell lysate, A549 cells, Human lung cancer or Human breast cancer tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2A2B7 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab204996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 46 kDa. | |
| Flow Cyt | 1/200 - 1/400. | |
| IHC-P | 1/200 - 1/1000. |
Target
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Relevance
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 2778 Human
- Omim: 139320 Human
- SwissProt: P63092 Human
- SwissProt: P84996 Human
- SwissProt: Q5JWF2 Human
- Unigene: 125898 Human
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Alternative names
- Adenylate cyclase stimulating G alpha protein antibody
- AHO antibody
- Alternative gene product encoded by XL exon antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G protein alpha S antibody [2A2B7] (ab204996)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded section) analysis of Human breast cancer tissue labeling G protein alpha with ab204996 at a 1/200 dilution. DAB staining used.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G protein alpha S antibody [2A2B7] (ab204996)](https://www.abcam.com/ps/products/204/ab204996/Images/ab204996-247895-anti-g-protein-alpha-antibody-2a2b7-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G protein alpha S antibody [2A2B7] (ab204996)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded section) analysis of Human lung cancer tissue labeling G protein alpha with ab204996 at a 1/200 dilution. DAB staining used.
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![Flow Cytometry - Anti-G protein alpha S antibody [2A2B7] (ab204996)](https://www.abcam.com/ps/products/204/ab204996/Images/ab204996-247894-anti-g-protein-alpha-antibody-2a2b7-flow-cytometry.jpg)
Flow Cytometry - Anti-G protein alpha S antibody [2A2B7] (ab204996)Flow cytometric analysis of A549 cells labelling Human G protein alpha with ab204996 (green) at a 1/200 dilution and negative control (red).
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![Western blot - Anti-G protein alpha S antibody [2A2B7] (ab204996)](https://www.abcam.com/ps/products/204/ab204996/Images/ab204996-247893-anti-g-protein-alpha-antibody-2a2b7-western-blot.jpg)
Western blot - Anti-G protein alpha S antibody [2A2B7] (ab204996)All lanes : Anti-G protein alpha S antibody [2A2B7] (ab204996) at 1/500 dilution
Lane 1 : HEK293 whole cell lysate
Lane 2 : G protein alpha (aa42-188)-hIgGFc transfected HEK293 whole cell lysate
Predicted band size: 46 kDa -
![Western blot - Anti-G protein alpha S antibody [2A2B7] (ab204996)](https://www.abcam.com/ps/products/204/ab204996/Images/ab204996-247892-anti-g-protein-alpha-antibody-2a2b7-western-blot.jpg)
Western blot - Anti-G protein alpha S antibody [2A2B7] (ab204996)Anti-G protein alpha S antibody [2A2B7] (ab204996) at 1/500 dilution + Human G protein alpha (aa42-188) recombinant protein
Predicted band size: 46 kDa
Additional bands at: 42.8 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
References (0)
ab204996 has not yet been referenced specifically in any publications.
