Key features and details
- Mouse monoclonal [7G6G5] to G protein alpha S
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-G protein alpha S antibody [7G6G5]
See all GNAS primary antibodies
DescriptionMouse monoclonal [7G6G5] to G protein alpha S
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human G protein alpha aa 42-188. Expressed in E. Coli.
Database link: P63092
- WB: Human G protein alpha (aa: 42-188) recombinant protein; Human G protein alpha (aa: 42-188)-hIgGFc transfected HEK293 cell lysate. ICC/IF: HeLa cells. FACS: MCF7 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab216129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 45 kDa.|
|ICC/IF||1/200 - 1/1000.|
|Flow Cyt||1/200 - 1/400.|
RelevanceGuanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
Cellular localizationCell Membrane
- Adenylate cyclase stimulating G alpha protein antibody
- AHO antibody
- Alternative gene product encoded by XL exon antibody
Flow cytometric analysis of MCF7 cells labeling G protein alpha with ab216129 at 1/200 dilution (green). Negative control (red).
Immunofluorescent analaysis of HeLa cells labeling G protein alpha with ab216129 at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
All lanes : Anti-G protein alpha S antibody [7G6G5] (ab216129) at 1/500 dilution
Lane 1 : non-transfected HEK293 cell lysate
Lane 2 : Human G protein alpha (aa: 42-188)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 45 kDa
Anti-G protein alpha S antibody [7G6G5] (ab216129) at 1/500 dilution + Human G protein alpha (aa: 42-188) recombinant protein
Predicted band size: 45 kDa
Expected MW is 42.8 kDa
ab216129 has not yet been referenced specifically in any publications.