Anti-G protein alpha S antibody [7G6G5] (ab216129)

Submit a review Submit a question
Flow Cytometry - Anti-G protein alpha S antibody [7G6G5] (ab216129)
  • Immunocytochemistry/ Immunofluorescence - Anti-G protein alpha S antibody [7G6G5] (ab216129)
  • Western blot - Anti-G protein alpha S antibody [7G6G5] (ab216129)
  • Western blot - Anti-G protein alpha S antibody [7G6G5] (ab216129)

Key features and details

  • Mouse monoclonal [7G6G5] to G protein alpha S
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-G protein alpha S antibody [7G6G5]
    See all GNAS primary antibodies

  • Description

    Mouse monoclonal [7G6G5] to G protein alpha S

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human G protein alpha aa 42-188. Expressed in E. Coli.
    Database link: P63092

  • Positive control

    • WB: Human G protein alpha (aa: 42-188) recombinant protein; Human G protein alpha (aa: 42-188)-hIgGFc transfected HEK293 cell lysate. ICC/IF: HeLa cells. FACS: MCF7 cells.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab216129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 45 kDa.
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

Target

  • Relevance

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.

  • Cellular localization

    Cell Membrane

  • Database links

    • Alternative names

      • Adenylate cyclase stimulating G alpha protein antibody
      • AHO antibody
      • Alternative gene product encoded by XL exon antibody
      • C20orf45 antibody
      • dJ309F20.1.1 antibody
      • dJ806M20.3.3 antibody
      • Extra large alphas protein antibody
      • GNAS antibody
      • GNAS complex locus antibody
      • GNAS1 antibody
      • GPSA antibody
      • Gs alpha subunit antibody
      • GSA antibody
      • GSP antibody
      • Guanine nucleotide binding protein (G protein) alpha stimulating activity polypeptide 1 antibody
      • Guanine nucleotide binding protein alpha stimulating activity polypeptide 1 antibody
      • Guanine nucleotide binding protein G(s) subunit alpha isoforms short antibody
      • Guanine nucleotide binding protein G(s) subunit alpha isoforms XLas antibody
      • Guanine nucleotide regulatory protein antibody
      • MGC33735 antibody
      • NESP antibody
      • NESP55 antibody
      • Neuroendocrine secretory protein antibody
      • PHP1A antibody
      • PHP1B antibody
      • PHP1C antibody
      • POH antibody
      • Protein ALEX antibody
      • Protein GNAS antibody
      • Protein NESP55 antibody
      • SCG6 antibody
      • Secretogranin VI antibody
      • XLalphas antibody
      • XLas antibody
      see all

    Images

    • Flow Cytometry - Anti-G protein alpha S antibody [7G6G5] (ab216129)
      Flow Cytometry - Anti-G protein alpha S antibody [7G6G5] (ab216129)

      Flow cytometric analysis of MCF7 cells labeling G protein alpha with ab216129 at 1/200 dilution (green). Negative control (red).

    • Immunocytochemistry/ Immunofluorescence - Anti-G protein alpha S antibody [7G6G5] (ab216129)
      Immunocytochemistry/ Immunofluorescence - Anti-G protein alpha S antibody [7G6G5] (ab216129)

      Immunofluorescent analaysis of HeLa cells labeling G protein alpha with ab216129 at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

    • Western blot - Anti-G protein alpha S antibody [7G6G5] (ab216129)
      Western blot - Anti-G protein alpha S antibody [7G6G5] (ab216129)
      All lanes : Anti-G protein alpha S antibody [7G6G5] (ab216129) at 1/500 dilution

      Lane 1 : non-transfected HEK293 cell lysate
      Lane 2 : Human G protein alpha (aa: 42-188)-hIgGFc transfected HEK293 cell lysate

      Predicted band size: 45 kDa

    • Western blot - Anti-G protein alpha S antibody [7G6G5] (ab216129)
      Western blot - Anti-G protein alpha S antibody [7G6G5] (ab216129)
      Anti-G protein alpha S antibody [7G6G5] (ab216129) at 1/500 dilution + Human G protein alpha (aa: 42-188) recombinant protein

      Predicted band size: 45 kDa



      Expected MW is 42.8 kDa

    References (0)

    Publishing research using ab216129? Please let us know so that we can cite the reference in this datasheet.

    ab216129 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab216129.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com