Anti-G protein alpha S antibody (ab101736)

DISCOUNT CODE:XXXXX Expiration date: I am very pleased to hear you would like to accept our offer and test ab101736 in ICC. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for ICC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thank you for contacting Abcam. Have you already purchased the product? If so, do you have the order number used? It is usually the case that the 100% Abreview discount must be created prior to purchase of the antibody to be tested. I look forward to your reply.

Thank you very much for your interest in ab101736. I would recommend that when starting this procedure that you use an ice cold methanol or acetone fixation. Fixation times can vary from 1-10 minutes so it may be best to do a series of different times. Be sure to thouroughly rinse the samples in ice cold PBS following fixation. The advantage of using methanol or acetone is that they will permeablize the cells allowing you to skipthis step later. I would also recommend using a blocking solution containing 1% BSA in PBST or a 10% serum of the animal your secondary antibody was raised in. Block for at least 30 minutes at room temperatures. You will have to titer out the correct dilution of antibody to use for your experiments however a good starting point is 5ug/mL. Incubate at room temperature for 1 hour or overnight at 4C. I would like to direct you to our protocols and tips page for further ideas and detailed protcols. You may find these under the Immunostaining headingin the lower left hand column. The link is: https://www.abcam.com/index.html?pageconfig=popular_protocols To our knowledge, ab101736 has not been tested in ICC. Therefore, I can offer a discount off a future purchase if you buy ab101736 now, test it in ICC and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab101736 in ICC. I will then send a discount code. This code must be issued before purchasing ab101736 so please wait for my reply before ordering. 2. Purchase ab101736 either by phone, fax, or online (www.abcam.com). 3. Test it in ICC. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab101736 turns out to be unsuitable for ICC, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Thank you very much for your interest in ab101736. This particular antibody has been tested in Western Blot but to our knowledge, ab101736 has not been tested in ICC. Therefore, I can offer a discount off a future purchase if you buy ab101736 now, test it inICC and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab101736 in ICC. I will then send a discount code. This code must be issued before purchasing ab101736 so please wait for my reply before ordering. 2. Purchase ab101736 either by phone, fax, or online (www.abcam.com). 3. Test it in ICC. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab101736 turns out to be unsuitable for ICC, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Thank you for contacting us. The Anti-G protein alpha S antibody ab101736 will not recognize G alpha olf or other G protein subunits. The immunizing peptide only matches with mouse GNAS NP_034439.2 and GNASL NP_963910.1 (both GeneID 14683). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.