- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-G protein alpha S antibody
See all G protein alpha S primary antibodies -
DescriptionGoat polyclonal to G protein alpha S
-
Host speciesGoat
-
Specificityab101736 is expected to recognize all three reported isoforms NP_000507.1, NP_001070956.1 and NP_536350.2.
-
Tested applicationsSuitable for: WBmore details
-
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Pig
-
Immunogen
Synthetic peptide:
C-QAARSNSDGEKATK
, corresponding to internal sequence amino acids 78-91 of Human G protein alpha S (NP_000507.1). -
Positive control
- Jurkat cell line lysate
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
-
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
-
Purification notesab101736 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab101736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa). |
Target
-
RelevanceGuanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
-
Cellular localizationCell Membrane
-
Database links
- Entrez Gene: 281793 Cow
- Entrez Gene: 2778 Human
- Entrez Gene: 14683 Mouse
- Entrez Gene: 100049657 Pig
- Entrez Gene: 24896 Rat
- Omim: 139320 Human
- SwissProt: P04896 Cow
- SwissProt: P63091 Dog
see all -
Alternative names
- Adenylate cyclase stimulating G alpha protein antibody
- AHO antibody
- Alternative gene product encoded by XL exon antibody
see all
Images
-
Western blot - Anti-G protein alpha S antibody (ab101736)Anti-G protein alpha S antibody (ab101736) at 1 µg/ml + Jurkat cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 46 kDa
Primary incubation was 1 hour. -
Western blot - Anti-G protein alpha S antibody (ab101736)All lanes : Anti-G protein alpha S antibody (ab101736) at 0.1 µg/ml
Lane 1 : Mouse Brain lysate in RIPA buffer
Lane 2 : Rat Brain lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 46 kDa
Protocols
References
ab101736 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"