Anti-G protein alpha S antibody (ab101736)
Key features and details
- Goat polyclonal to G protein alpha S
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-G protein alpha S antibody
See all GNAS primary antibodies -
Description
Goat polyclonal to G protein alpha S -
Host species
Goat -
Specificity
ab101736 is expected to recognize all three reported isoforms NP_000507.1, NP_001070956.1 and NP_536350.2. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Pig -
Immunogen
Synthetic peptide corresponding to Human G protein alpha S aa 78-91 (internal sequence).
Sequence:C-QAARSNSDGEKATK
Database link: NP_000507.1 -
Positive control
- WB: Jurkat cell lysate and mouse and rat brain tissue lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab101736 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab101736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Relevance
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 281793 Cow
- Entrez Gene: 2778 Human
- Entrez Gene: 14683 Mouse
- Entrez Gene: 100049657 Pig
- Entrez Gene: 24896 Rat
- Omim: 139320 Human
- SwissProt: P04896 Cow
- SwissProt: P63091 Dog
see all -
Alternative names
- Adenylate cyclase stimulating G alpha protein antibody
- AHO antibody
- Alternative gene product encoded by XL exon antibody
see all
Images
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Anti-G protein alpha S antibody (ab101736) at 1 µg/ml + Jurkat cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 46 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. -
All lanes : Anti-G protein alpha S antibody (ab101736) at 0.1 µg/ml
Lane 1 : Mouse brain tissue lysate in RIPA buffer
Lane 2 : Rat brain tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 46 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected using chemiluminescence.
Datasheets and documents
References (0)
ab101736 has not yet been referenced specifically in any publications.