Anti-G protein beta subunit like antibody (ab25974)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-G protein beta subunit like antibody
See all G protein beta subunit like primary antibodies -
DescriptionRabbit polyclonal to G protein beta subunit like
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Host speciesRabbit
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Specificityab25974 recognises GßL (G protein beta protein subunit-like).
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, ELISAmore details
Unsuitable for: IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide, corresponding to 14 C terminal amino acids of Human G protein beta subunit like
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Positive control
- Human brain cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab25974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use a concentration of 1 µg/ml. Can be blocked with Human G protein beta subunit like peptide (ab39766). | |
IHC-P | Use a concentration of 10 µg/ml. | |
ICC/IF | Use a concentration of 20 µg/ml. | |
ELISA | Use at an assay dependent concentration. |
Target
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FunctionSubunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
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Tissue specificityBroadly expressed, with highest levels in skeletal muscle, heart and kidney.
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Sequence similaritiesBelongs to the WD repeat LST8 family.
Contains 7 WD repeats. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 64223 Human
- Entrez Gene: 56716 Mouse
- Entrez Gene: 64226 Rat
- Omim: 612190 Human
- SwissProt: Q9BVC4 Human
- SwissProt: Q9DCJ1 Mouse
- SwissProt: Q9Z2K5 Rat
- Unigene: 29203 Human
see all -
Alternative names
- G protein beta subunit like antibody
- G protein beta subunit-like antibody
- Gable antibody
see all
Images
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Lane 1 : Anti-G protein beta subunit like antibody (ab25974) at 1 µg/ml
Lane 2 : Anti-G protein beta subunit like antibody (ab25974) at 2 µg/ml
All lanes : Human brain cell lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G protein beta subunit like antibody (ab25974)
ab25974 at 10µg/ml staining G protein beta subunit like in mouse brain tissue by IHC
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Immunofluorescence of GBL in Mouse Brain cells using ab25974 at 10 ug/ml.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Lin Z et al. Construction of competitive endogenous RNA network reveals regulatory role of long non-coding RNAs in type 2 diabetes mellitus. J Cell Mol Med 21:3204-3213 (2017). Read more (PubMed: 28643459) »