Overview

  • Product name

    Anti-G protein beta subunit like/GBL antibody
    See all G protein beta subunit like/GBL primary antibodies
  • Description

    Rabbit polyclonal to G protein beta subunit like/GBL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant fragment within Human G protein beta subunit like/GBL (internal sequence). The exact sequence is proprietary.
    Database link: Q9BVC4

  • Positive control

    • WB: HeLa cell extract; HEK-293T whole cell lysate.
  • General notes

     This product was previously labelled as G protein beta subunit like

     

Properties

Applications

Our Abpromise guarantee covers the use of ab228832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.

Target

  • Function

    Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
  • Tissue specificity

    Broadly expressed, with highest levels in skeletal muscle, heart and kidney.
  • Sequence similarities

    Belongs to the WD repeat LST8 family.
    Contains 7 WD repeats.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • G protein beta subunit like antibody
    • G protein beta subunit-like antibody
    • Gable antibody
    • GbetaL antibody
    • GBL antibody
    • GBL protein antibody
    • LST8 antibody
    • LST8_HUMAN antibody
    • Mammalian lethal with SEC13 protein 8 antibody
    • MGC111011 antibody
    • mLST8 antibody
    • MTOR associated protein LST8 homolog (S. cerevisiae) antibody
    • POP3 antibody
    • POP3 homolog (S. pombe) antibody
    • Protein GbetaL antibody
    • Target of rapamycin complex subunit LST8 antibody
    • TORC subunit LST8 antibody
    • WAT1 antibody
    • WAT1 homolog (S. pombe) antibody
    see all

Images

  • All lanes : Anti-G protein beta subunit like/GBL antibody (ab228832) at 1/500 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : G protein beta subunit like Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 36 kDa

  • All lanes : Anti-G protein beta subunit like/GBL antibody (ab228832) at 1/500 dilution

    Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : G protein beta subunit like knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 36 kDa



    10% SDS-PAGE gel.

  • Anti-G protein beta subunit like/GBL antibody (ab228832) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Predicted band size: 36 kDa



    10% SDS-PAGE gel.

References

ab228832 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228832.
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