Overview

  • Product name

    Anti-G-protein coupled receptor 30 antibody
    See all G-protein coupled receptor 30 primary antibodies
  • Description

    Rabbit polyclonal to G-protein coupled receptor 30
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 244-336 of Human G-protein coupled receptor 30 (Uniprot: Q99527).

  • Positive control

    • HepG2 membrane lysate; Human SNU1 xenograft tissue.
  • General notes

    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab137479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 42 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Receptor for estrogen.
  • Tissue specificity

    Ubiquitously expressed, but is most abundant in placenta. In brain regions, expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Protein has been detected in the cell membrane, endoplasmic reticulum and Golgi apparatus. It is currently unclear whether this is a cell surface or intracellular receptor.
  • Information by UniProt
  • Database links

  • Alternative names

    • CEPR antibody
    • Chemoattractant receptor-like 2 antibody
    • Chemokine receptor-like 2 antibody
    • CMKRL2 antibody
    • Constitutively expressed peptide like receptor antibody
    • DRY12 antibody
    • FEG 1 antibody
    • FEG-1 antibody
    • Flow-induced endothelial G protein-coupled receptor antibody
    • Flow-induced endothelial G-protein coupled receptor 1 antibody
    • G protein-coupled receptor 30 antibody
    • G-protein coupled estrogen receptor 1 antibody
    • G-protein coupled receptor 30 antibody
    • GPCR-BR antibody
    • Gper antibody
    • GPER_HUMAN antibody
    • GPER1 antibody
    • GPR30 antibody
    • IL8-related receptor DRY12 antibody
    • Lergu antibody
    • LERGU2 antibody
    • leucine rich protein in GPR30 3'UTR antibody
    • LYGPR antibody
    • Lymphocyte-derived G-protein coupled receptor antibody
    • Membrane estrogen receptor antibody
    • mER antibody
    • MGC99678 antibody
    see all

Images

  • Anti-G-protein coupled receptor 30 antibody (ab137479) at 1/500 dilution + HepG2 membrane lysate
    at 30 µg

    Predicted band size: 42 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human SNU1 xenograft tissue labelling G-protein coupled receptor 30 with ab137479 antibody at 1/100 dilution.

References

This product has been referenced in:

  • Vivacqua A  et al. Differential MicroRNA Landscape Triggered by Estrogens in Cancer Associated Fibroblasts (CAFs) of Primary and Metastatic Breast Tumors. Cancers (Basel) 11:N/A (2019). Read more (PubMed: 30909585) »
  • Santolla MF  et al. GPER Mediates a Feedforward FGF2/FGFR1 Paracrine Activation Coupling CAFs to Cancer Cells toward Breast Tumor Progression. Cells 8:N/A (2019). Read more (PubMed: 30866584) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Total proteins from testicle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
15 µg
Specification
Total proteins from testicle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 19 2018

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