Anti-G-protein coupled receptor 30 antibody (ab39742)


  • Product name
    Anti-G-protein coupled receptor 30 antibody
    See all G-protein coupled receptor 30 primary antibodies
  • Description
    Rabbit polyclonal to G-protein coupled receptor 30
  • Host species
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human GPCR GPR30.

    (Peptide available as ab41565.)

  • Positive control
    • ab39742 gives a positive signal in Human Brain Membrane Lysate, Human Brain Tissue Lysate (data not shown), and MCF7 Whole Cell Lysate (data not shown) This antibody gave a positive signal in the following cell types: U-87 MG.



Our Abpromise guarantee covers the use of ab39742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200 - 1/400.
WB 1/250. Detects a band of approximately 55 kDa (predicted molecular weight: 42 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 24421881


  • Function
    Receptor for estrogen.
  • Tissue specificity
    Ubiquitously expressed, but is most abundant in placenta. In brain regions, expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Protein has been detected in the cell membrane, endoplasmic reticulum and Golgi apparatus. It is currently unclear whether this is a cell surface or intracellular receptor.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEPR antibody
    • Chemoattractant receptor-like 2 antibody
    • Chemokine receptor-like 2 antibody
    • CMKRL2 antibody
    • Constitutively expressed peptide like receptor antibody
    • DRY12 antibody
    • FEG 1 antibody
    • FEG-1 antibody
    • Flow-induced endothelial G protein-coupled receptor antibody
    • Flow-induced endothelial G-protein coupled receptor 1 antibody
    • G protein-coupled receptor 30 antibody
    • G-protein coupled estrogen receptor 1 antibody
    • G-protein coupled receptor 30 antibody
    • GPCR-BR antibody
    • Gper antibody
    • GPER_HUMAN antibody
    • GPER1 antibody
    • GPR30 antibody
    • IL8-related receptor DRY12 antibody
    • Lergu antibody
    • LERGU2 antibody
    • leucine rich protein in GPR30 3'UTR antibody
    • LYGPR antibody
    • Lymphocyte-derived G-protein coupled receptor antibody
    • Membrane estrogen receptor antibody
    • mER antibody
    • MGC99678 antibody
    see all


  • ab39742 staining cultured rat primary astrocytes by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured cells were then stained with ab39742 at 1/500 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue. In red astrocytes can be observed (monoclonal anti GFAP).

    See Abreview

  • ICC/IF image of ab39742 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab39742 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-G-protein coupled receptor 30 antibody (ab39742) at 1 µg/ml + Human brain normal tissue lysate - membrane extract (ab29456) at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 55 kDa (why is the actual band size different from the predicted?)

    GPR30 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Immunohistochemical analysis of Human ovarian endometriosis tissue, staining G-protein coupled receptor 30 with ab39742 at 1/50 dilution.


This product has been referenced in:
  • Yan L  et al. Effects of Ovariectomy in an hSOD1-G93A Transgenic Mouse Model of Amyotrophic Lateral Sclerosis (ALS). Med Sci Monit 24:678-686 (2018). Read more (PubMed: 29394243) »
  • Wu Y  et al. Downregulation of G-protein-coupled receptor 30 in the hippocampus attenuates the neuroprotection of estrogen in the critical period hypothesis. Mol Med Rep 17:5716-5725 (2018). Read more (PubMed: 29484405) »

See all 17 Publications for this product

Customer reviews and Q&As

Flow Cytometry
Rat Cell (Lung tissue)
Yes - eBiosciences FoxP3 intracellular staining buffer set
Gating Strategy
Lung tissue
Cell harvesting/tissue preparation method: Lungs were dissected and digested using Collagenase IV+DNase
Sample buffer: GKN+10% FCS
eBiosciences FoxP3 intracellular staining buffer set

Jeff Lauzon-Joset

Verified customer

Submitted Jan 12 2016

Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Human Cell lysate - whole cell (Breast -MCF7)
Breast -MCF7
1nM estradiol 2-24 hours
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 08 2013

Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
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He llevado a cabo una búsqueda en nuestro catálogo de anticuerpos contra los receptores indicados:

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Rat Cell (Primary Astrocytes culture)
Primary Astrocytes culture
Yes - 0.1% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10%

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 13 2012

Thank you for contacting us.  We currently have three antibodies that recognize GPCR GPR30: ab39742 - rabbit polyclonal; reacts with: human, mouse, and rat; tested for IHC-Fr and WB ab118512 - goat polyclonal; reacts with human and predicted to react w...

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1-10 of 16 Abreviews or Q&A


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