Overview

  • Product name
    Anti-G-protein coupled receptor 30 antibody
    See all G-protein coupled receptor 30 primary antibodies
  • Description
    Rabbit polyclonal to G-protein coupled receptor 30
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human GPCR GPR30.

    Read Abcam's proprietary immunogen policy (Peptide available as ab41565.)

  • Positive control
    • ab39742 gives a positive signal in Human Brain Membrane Lysate, Human Brain Tissue Lysate (data not shown), and MCF7 Whole Cell Lysate (data not shown) This antibody gave a positive signal in the following cell types: U-87 MG.

Properties

Applications

Our Abpromise guarantee covers the use of ab39742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200 - 1/400.
WB 1/250. Detects a band of approximately 55 kDa (predicted molecular weight: 42 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 24421881

Target

  • Function
    Receptor for estrogen.
  • Tissue specificity
    Ubiquitously expressed, but is most abundant in placenta. In brain regions, expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Protein has been detected in the cell membrane, endoplasmic reticulum and Golgi apparatus. It is currently unclear whether this is a cell surface or intracellular receptor.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEPR antibody
    • Chemoattractant receptor-like 2 antibody
    • Chemokine receptor-like 2 antibody
    • CMKRL2 antibody
    • Constitutively expressed peptide like receptor antibody
    • DRY12 antibody
    • FEG 1 antibody
    • FEG-1 antibody
    • Flow-induced endothelial G protein-coupled receptor antibody
    • Flow-induced endothelial G-protein coupled receptor 1 antibody
    • G protein-coupled receptor 30 antibody
    • G-protein coupled estrogen receptor 1 antibody
    • G-protein coupled receptor 30 antibody
    • GPCR-BR antibody
    • Gper antibody
    • GPER_HUMAN antibody
    • GPER1 antibody
    • GPR30 antibody
    • IL8-related receptor DRY12 antibody
    • Lergu antibody
    • LERGU2 antibody
    • leucine rich protein in GPR30 3'UTR antibody
    • LYGPR antibody
    • Lymphocyte-derived G-protein coupled receptor antibody
    • Membrane estrogen receptor antibody
    • mER antibody
    • MGC99678 antibody
    see all

Images

  • ab39742 staining cultured rat primary astrocytes by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured cells were then stained with ab39742 at 1/500 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue. In red astrocytes can be observed (monoclonal anti GFAP).

    See Abreview

  • ICC/IF image of ab39742 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab39742 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-G-protein coupled receptor 30 antibody (ab39742) at 1 µg/ml + Human brain normal tissue lysate - membrane extract (ab29456) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?



    GPR30 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Immunohistochemical analysis of Human ovarian endometriosis tissue, staining G-protein coupled receptor 30 with ab39742 at 1/50 dilution.

References

This product has been referenced in:
  • Yan L  et al. Effects of Ovariectomy in an hSOD1-G93A Transgenic Mouse Model of Amyotrophic Lateral Sclerosis (ALS). Med Sci Monit 24:678-686 (2018). Read more (PubMed: 29394243) »
  • Wu Y  et al. Downregulation of G-protein-coupled receptor 30 in the hippocampus attenuates the neuroprotection of estrogen in the critical period hypothesis. Mol Med Rep 17:5716-5725 (2018). Read more (PubMed: 29484405) »
See all 19 Publications for this product

Customer reviews and Q&As

1-10 of 16 Abreviews or Q&A

Application
Flow Cytometry
Sample
Rat Cell (Lung tissue)
Permeabilization
Yes - eBiosciences FoxP3 intracellular staining buffer set
Gating Strategy
Leukocytes
Specification
Lung tissue
Preparation
Cell harvesting/tissue preparation method: Lungs were dissected and digested using Collagenase IV+DNase
Sample buffer: GKN+10% FCS
Fixation
eBiosciences FoxP3 intracellular staining buffer set

Jeff Lauzon-Joset

Verified customer

Submitted Jan 12 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Breast -MCF7)
Specification
Breast -MCF7
Treatment
1nM estradiol 2-24 hours
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 08 2013

Answer

Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
In order to understand the problems encountered more fully and be able to offer any tips which may improve the results observed so far, would you mind answering the following questions:
1. Sample preparation: Lysis buffer: Protease inhibitors: Reducing agent: Heated prior to loading on gel? Temperature/time:
2. Was a loading control used?
3. Which secondary antibody is being used? Has this been used with any other primary antibody before? What have been the results of this?
4. Order number (or if you do not have access to this the approximate delivery date and the delivery address used).
This information will also allow us to investigate this case internally and initiate additional testing where necessary.
If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
I look forward to receiving your reply.

Read More

Answer

Thank you for contacting us. Can you tell me how your oocyte samples were fixed and embedded? Were you using fluorescent or colorimetric detection? Fluorescent signal can easily fade after storage for a few days, whereas colorimetric signal will be stable for months to years at room temperature, provided that the samples are properly fixed and embedded. How were you storing your samples? Were they mounted to a slide and coverslipped, or were they in solution? The signal should be more stable when the samples are mounted. I hope this helps, please let me know if you need any additional information or assistance.

Read More

Answer

Thank you for contacting us.

My Spanish colleague is currently out of the office.

About the ER alpha R andER beta R antibodies:
The antibodies Ariana suggested to you are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

About the anti-Mas (https://www.abcam.com/ab123326 and https://www.abcam.com/ab66030) and the anti-GPER R (https://www.abcam.com/ab39742):
To our knowledge,these antibodieshave not been tested in IP.
Therefore, I can offer a discount off a future purchase if you buythese antibodiesnow, testthem inIP and submit feedback to us in the form of an Abreview.
It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback.
The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know which antibody you would like tobuy and test in IP. I will then send a discount code. This code must be issued before purchasingthe antibodies you want to test,so please wait for my reply before ordering.

2. Purchasethe antibody you want to testeither by phone, fax, or online (https://www.abcam.com).

3. Test it/them in IP.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe tested antibodyturns out to be unsuitable for IP, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

Read More

Answer

Gracias por contactarnos.



He llevado a cabo una búsqueda en nuestro catálogo de anticuerpos contra los receptores indicados:



-Para MAS, tenemos dos opciones, pero lamentablemente ninguna de ellas se ha validado mediante IP, pero si en WB. Si queréis echar un vistazo a sus datasheets adjunto el enlace a ellas:



https://www.abcam.com/MAS1-antibody-ab123326.html



https://www.abcam.com/MAS1-antibody-ab66030.html



-Contra GPER, también hay dos anticuerpos disponibles, aunque me temo que tampoco se han usado en IP. Uno de ellos sin embargo se ha validado en WB:



https://www.abcam.com/G-protein-coupled-receptor-30-antibody-ab39742.html



-Contra ER alpha Receptor, hay varios anticuerpos validados mediante IP con células humanas, podéis echar un vistazo a las distintas alternativas en el siguiente enlace:



https://www.abcam.com/index.html?pageconfig=searchresults&search=ER%20alpha%20Receptor&pt=1&sk=app&sv=70&sn=IP&l=3&fViewMore=1



-Por último, para Estrogen Receptor beta, también hay dos alternativas validadas en IP con células humanas:



https://www.abcam.com/Estrogen-Receptor-beta-antibody-988-ab16813.html



https://www.abcam.com/Estrogen-Receptor-beta-antibody-EPR3777-ab92306.html






Siento que para los dos primeros receptores no haya una alternativa adecuada, sin embargo si queréis, podéis participar en nuestro sistema de evaluación de anticuerpos Abreview.

En Abcam ofrecemos un servicio de evaluación de anticuerpos, llamado Abreview, por el cual podemos ofrecerte un descuento (por valor de un anticuerpo primario / proteína) para una futura compra si adquieres el producto y lo testas en una aplicación o en una especie no testada hasta ahora (como IP), y publicas los resultados en nuestra website, en forma de Abreview, independientemente de que éstos sean positivos o negativos.

Si estás interesado en la oferta, házmelo saber antes de comprar los anticuerpos, para que te envie el código canjeable por un anticuerpo gratuito en la futura compra.

Para conocer con más detalle nuestro sistema de Abreview, visita la página: https://www.abcam.com/abreview.

No dudes en ponerte en contacto con nosotros si tienes cualquier otra consulta sobre esta oferta y estaré encantada de poder ayudarte. Si necesitas un presupuesto con alguno de los productos mencionados, también estaré encantada de enviártelo.

Read More

Answer

Thank you for getting back to me regarding this case.



I have gone ahead and arranged for ab52650 Anti-PTP1B antibody [EP1837Y]to be shipped to you using the same purchasing route as before. We currently do not have local stock and our overseas stock will only reach you some time next week.Please also inform yourlocal sub-dealer (宮崎化学品株式会社) that you are expecting this free of charge product from us so that they too will be looking out for it. (Order # 1113473)



If there is anything else that I can help you with please do not hesitate to contact me.



Have a nice day!

Read More

Answer

Thank you for your feedback.


I am sorry to hear that the different lot replacement vial also did not work as you hoped. Should you be interested in a different target primary antibody from our catalogue, I will be more than happy to offer you a free primary antibody of your choice. Simply inform me the catalogue number and I will arrange for the product to be shipped to you.


I hope this one incident will not hinderyou from considering us again as your source of antibodies and reagents in the future.


Thank you and good luck in your research.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary Astrocytes culture)
Specification
Primary Astrocytes culture
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10%

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 13 2012

Question
Answer

Thank you for contacting us.  We currently have three antibodies that recognize GPCR GPR30: ab39742 - rabbit polyclonal; reacts with: human, mouse, and rat; tested for IHC-Fr and WB ab118512 - goat polyclonal; reacts with human and predicted to react with mouse and rat; tested for IHC-P ab98075 - rabbit polyclonal; reacts with human; tested for Flow Cyt, ICC/IF, and IHC-P. I hope this helps, please let me know if you need any additional information. 

Read More

1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up