Anti-G-protein coupled receptor 30 antibody (ab39742)

Rabbit polyclonal G-protein coupled receptor 30 antibody. Validated in WB, IHC, ICC/IF and tested in Mouse, Rat, Human. Cited in 27 publication(s). Independently reviewed in 7 review(s).

Overview

  • Product name
    Anti-G-protein coupled receptor 30 antibody
    See all G-protein coupled receptor 30 primary antibodies
  • Description
    Rabbit polyclonal to G-protein coupled receptor 30
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human GPCR GPR30.

    Read Abcam's proprietary immunogen policy (Peptide available as ab41565.)

  • Positive control
    • ab39742 gives a positive signal in Human Brain Membrane Lysate, Human Brain Tissue Lysate (data not shown), and MCF7 Whole Cell Lysate (data not shown) This antibody gave a positive signal in the following cell types: U-87 MG.

Properties

Applications

Our Abpromise guarantee covers the use of ab39742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200 - 1/400.
WB 1/250. Detects a band of approximately 55 kDa (predicted molecular weight: 42 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 24421881

Target

  • Function
    Receptor for estrogen.
  • Tissue specificity
    Ubiquitously expressed, but is most abundant in placenta. In brain regions, expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Protein has been detected in the cell membrane, endoplasmic reticulum and Golgi apparatus. It is currently unclear whether this is a cell surface or intracellular receptor.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEPR antibody
    • Chemoattractant receptor-like 2 antibody
    • Chemokine receptor-like 2 antibody
    • CMKRL2 antibody
    • Constitutively expressed peptide like receptor antibody
    • DRY12 antibody
    • FEG 1 antibody
    • FEG-1 antibody
    • Flow-induced endothelial G protein-coupled receptor antibody
    • Flow-induced endothelial G-protein coupled receptor 1 antibody
    • G protein-coupled receptor 30 antibody
    • G-protein coupled estrogen receptor 1 antibody
    • G-protein coupled receptor 30 antibody
    • GPCR-BR antibody
    • Gper antibody
    • GPER_HUMAN antibody
    • GPER1 antibody
    • GPR30 antibody
    • IL8-related receptor DRY12 antibody
    • Lergu antibody
    • LERGU2 antibody
    • leucine rich protein in GPR30 3'UTR antibody
    • LYGPR antibody
    • Lymphocyte-derived G-protein coupled receptor antibody
    • Membrane estrogen receptor antibody
    • mER antibody
    • MGC99678 antibody
    see all

Images

  • Anti-G-protein coupled receptor 30 antibody (ab39742) at 1 µg/ml + Human brain normal tissue lysate - membrane extract (ab29456) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?



    GPR30 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • ab39742 staining cultured rat primary astrocytes by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured cells were then stained with ab39742 at 1/500 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue. In red astrocytes can be observed (monoclonal anti GFAP).

    See Abreview

  • ICC/IF image of ab39742 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab39742 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of Human ovarian endometriosis tissue, staining G-protein coupled receptor 30 with ab39742 at 1/50 dilution.

References

This product has been referenced in:
  • Gogola J  et al. Persistent endocrine-disrupting chemicals found in human follicular fluid stimulate the proliferation of granulosa tumor spheroids via GPR30 and IGF1R but not via the classic estrogen receptors. Chemosphere 217:100-110 (2019). Read more (PubMed: 30414542) »
  • Cortes E  et al. GPER is a mechanoregulator of pancreatic stellate cells and the tumor microenvironment. EMBO Rep 20:N/A (2019). Read more (PubMed: 30538117) »
See all 29 Publications for this product

Customer reviews and Q&As

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1-7 of 7 Abreviews

Application
Flow Cytometry
Sample
Rat Cell (Lung tissue)
Permeabilization
Yes - eBiosciences FoxP3 intracellular staining buffer set
Gating Strategy
Leukocytes
Specification
Lung tissue
Preparation
Cell harvesting/tissue preparation method: Lungs were dissected and digested using Collagenase IV+DNase
Sample buffer: GKN+10% FCS
Fixation
eBiosciences FoxP3 intracellular staining buffer set

Jeff Lauzon-Joset

Verified customer

Submitted Jan 12 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Breast -MCF7)
Specification
Breast -MCF7
Treatment
1nM estradiol 2-24 hours
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary Astrocytes culture)
Specification
Primary Astrocytes culture
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10%

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (muscle)
Loading amount
40 µg
Specification
muscle
Gel Running Conditions
Non-reduced Denaturing (12% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 15 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (whole brain)
Loading amount
10 µg
Specification
whole brain
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted May 01 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Cow Cell lysate - other (liver)
Loading amount
45 µg
Specification
liver
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 10 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (brain sections)
Specification
brain sections
Fixative
Paraformaldehyde
Permeabilization
No

Dr. Sophie Pezet

Verified customer

Submitted Jul 25 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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