Question (65762) | Anti-G-protein coupled receptor 30 antibody (ab39742)

Go to datasheet (ab39742)

Question

Product code: 39742
Lot number: GR78499-1
Inquiry: Got multiple bands with this antibody 20 and 50ug of whoel cell lysate from MCF7 and RI-1 cells Blocking: 5% milk 2 h primary antibody: 1:1000 ON at 4deg in TBS-T 3 times 10' wash in TBS-T secondary antibody: 1:5000 2h at RT in TBS-T 3 times 10' wash in TBS-T ECL by Thermofisher Any suggestions? Would a replacement be possible? Best wishes

Answer

Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
In order to understand the problems encountered more fully and be able to offer any tips which may improve the results observed so far, would you mind answering the following questions:
1. Sample preparation: Lysis buffer: Protease inhibitors: Reducing agent: Heated prior to loading on gel? Temperature/time:
2. Was a loading control used?
3. Which secondary antibody is being used? Has this been used with any other primary antibody before? What have been the results of this?
4. Order number (or if you do not have access to this the approximate delivery date and the delivery address used).
This information will also allow us to investigate this case internally and initiate additional testing where necessary.
If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
I look forward to receiving your reply.

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