Serum as blocking agent for 30 minute(s) · Concentration: 10%
Other product details
4 hour(s) and 0 minute(s) · Temperature: 24°C
Non-Abcam antibody was used: anti rabbit Alexa Fluro 488 Host species: Donkey Clonality: Polyclonal Conjugation: Alexa Fluor® 488
ICC/IF image of (Ab39742): Cultured astrocytes were stained with polyclonal anti-GPCR GPR30. The cells were 4% formaldehyde fixed (5min) and then incubated in 10% normal donkeys serum in 0.1% PBS-0.3% TritonX for 30 minutes to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Ab66048, 1/500 dilution) 4 hours at 24°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution and monoclonal antiGFAP-568for 1h. Hoechst was used to stain the cell nuclei (blue) at a concentration of 1.43µM. GPCR expressed in selected astrocytes.
Dr. Ruma Raha-Chowdhury
Submitted Feb 13 2012
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