Anti-G3BP antibody [2F3] (ab56574)
![Immunocytochemistry/ Immunofluorescence - Anti-G3BP antibody [2F3] (ab56574)](https://www.abcam.com/ps/products/56/ab56574/Images/ab56574-69955-anti-g3bp-antibody-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-G3BP antibody [2F3] (ab56574)")
Key features and details
- Mouse monoclonal [2F3] to G3BP
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-G3BP antibody [2F3]
See all G3BP primary antibodies -
Description
Mouse monoclonal [2F3] to G3BP -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment: KPEPVLEETA PEDAQKSSSP APADIAQTVQ EDLRTFSWAS VTSKNLPPSG AVPVTGIPPH VVKVPASQPR PESKPESQIP PQRPQRDQRV , corresponding to amino acids 214-303 of Human G3BP
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General notes
This product was changed from ascites to tissue culture supernatant on 22/03/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from tissue culture supernatant -
Clonality
Monoclonal -
Clone number
2F3 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab56574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| ICC/IF | (4) |
Use at an assay dependent concentration.
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| Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa. |
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IHC-P
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 NTF2 domain.
Contains 1 RRM (RNA recognition motif) domain. -
Domain
The NTF2 domain mediates multimerization. -
Post-translational
modificationsPhosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
Arg-435 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Cytoplasm. Cytoplasm > cytosol. Cell membrane. Nucleus. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS. - Information by UniProt
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Database links
- Entrez Gene: 10146 Human
- Omim: 608431 Human
- SwissProt: Q13283 Human
- Unigene: 587054 Human
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Alternative names
- ATP dependent DNA helicase VIII antibody
- ATP-dependent DNA helicase VIII antibody
- G3BP antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-G3BP antibody [2F3] (ab56574)
ICC/IF image of ab56574 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56574, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP antibody [2F3] (ab56574)](https://www.abcam.com/ps/products/56/ab56574/Images/ab56574-69957-anti-g3bp-antibody-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP antibody [2F3] (ab56574)G3BP antibody (ab56574) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human lymphoma.
This image was generated using the ascites version of the product.
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![Western blot - Anti-G3BP antibody [2F3] (ab56574)](https://www.abcam.com/ps/products/56/ab56574/Images/ab56574-69956-anti-g3bp-antibody-western-blot.jpg)
Western blot - Anti-G3BP antibody [2F3] (ab56574)G3BP antibody (ab56574) at 1ug/lane + A-431 cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-G3BP antibody [2F3] (ab56574)](https://www.abcam.com/ps/products/56/ab56574/Images/ab56574-69954-anti-g3bp-antibody-flow-cytometry.jpg)
Flow Cytometry - Anti-G3BP antibody [2F3] (ab56574)Overlay histogram showing HeLa cells stained with ab56574 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56574, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-G3BP antibody [2F3] (ab56574)](https://www.abcam.com/ps/products/56/ab56574/Images/ab56574-204918-anti-g3bp-antibody-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-G3BP antibody [2F3] (ab56574)This image is courtesy of an anonymous Abreviewab56574 staining G3BP in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 12 hours at 4°C. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 37°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
Top row - untreated cells. Bottom row - cells treated with sodium arsenite. Left - G3BP, Middle - Nucleus, Right - Merge.
Stress granules are visible in cells treated with sodium arsenite, whereas G3BP is dispersed in the cytoplasm in untreated cells.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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Datasheet download
References (52)
ab56574 has been referenced in 52 publications.
- Lu X et al. Role of Chikungunya nsP3 in Regulating G3BP1 Activity, Stress Granule Formation and Drug Efficacy. Arch Med Res 52:48-57 (2021). PubMed: 33131924
- Matheny T et al. RNA partitioning into stress granules is based on the summation of multiple interactions. RNA 27:174-189 (2021). PubMed: 33199441
- Lu S et al. The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein. Nat Commun 12:502 (2021). PubMed: 33479198
- Spijkers XM et al. A directional 3D neurite outgrowth model for studying motor axon biology and disease. Sci Rep 11:2080 (2021). PubMed: 33483540
- Gao B et al. Inhibition of anti-viral stress granule formation by coronavirus endoribonuclease nsp15 ensures efficient virus replication. PLoS Pathog 17:e1008690 (2021). PubMed: 33635931
