Immunocytochemistry/ Immunofluorescence abreview for Anti-G3BP antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample
Human Cell (Hela Cells)
Specification
Hela Cells
Permeabilization
Yes - Triton x100
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: PBS
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: goat anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
Hela cells were used for the experiment. Stress Granules are visible in cells treated with Sodium Arsenite, whereas G3BP1 is dispersed in cell cytoplasm in untreated Hela cells.

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Submitted Aug 23 2013

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