Overview

  • Product name
    Anti-G3BP antibody [EPR13985(B)]
    See all G3BP primary antibodies
  • Description
    Rabbit monoclonal [EPR13985(B)] to G3BP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human G3BP aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13283

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Hela, Jurkat, 293 and Ramos cell lysates; Jurkat cells, 293 cells, Human muscle tissue and Human colon tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 68 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IP 1/20 - 1/40.

Target

  • Function
    May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 1 NTF2 domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Domain
    The NTF2 domain mediates multimerization.
  • Post-translational
    modifications
    Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
    Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytosol. Cell membrane. Nucleus. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.
  • Information by UniProt
  • Database links
    • Alternative names
      • ATP dependent DNA helicase VIII antibody
      • ATP-dependent DNA helicase VIII antibody
      • G3BP antibody
      • G3BP stress granule assembly factor 1 antibody
      • G3BP-1 antibody
      • G3bp1 antibody
      • G3BP1_HUMAN antibody
      • GAP binding protein antibody
      • GAP SH3 domain binding protein 1 antibody
      • GAP SH3 domain-binding protein 1 antibody
      • GTPase activating protein (SH3 domain) binding protein 1 antibody
      • hDH VIII antibody
      • Human DNA helicase VIII antibody
      • MGC111040 antibody
      • Ras GTPase activating protein binding protein 1 antibody
      • Ras GTPase activating protein SH3 domain binding protein antibody
      • Ras GTPase-activating protein-binding protein 1 antibody
      • RasGAP associated endoribonuclease G3BP antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-G3BP antibody [EPR13985(B)] (ab181149)
      Immunocytochemistry/ Immunofluorescence - Anti-G3BP antibody [EPR13985(B)] (ab181149)

      Immunocytochemistry/Immunofluorescence analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) labeling G3BP with Purified ab181149 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • Immunohistochemistry paraffin embedded sections - Anti-G3BP antibody [EPR13985(B)] (ab181149)
      Immunohistochemistry paraffin embedded sections - Anti-G3BP antibody [EPR13985(B)] (ab181149)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue staining G3BP using ab181149 at 1/100 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody with Hematoxylin counterstain.

    • Western blot - Anti-G3BP antibody [EPR13985(B)] (ab181149)
      Western blot - Anti-G3BP antibody [EPR13985(B)] (ab181149)
      All lanes : Anti-G3BP antibody [EPR13985(B)] (ab181149) at 1/20000 dilution

      Lane 1 : Hela cell lysate with NFDM/TBST
      Lane 2 : Jurkat cell lysate with NFDM/TBST
      Lane 3 : Ramos cell lysate with NFDM/TBST
      Lane 4 : 293 cell lysate with NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Blocking peptides at 5 % per lane.

      Secondary
      All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 52 kDa
      Observed band size: 68 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemistry paraffin embedded sections - Anti-G3BP antibody [EPR13985(B)] (ab181149)
      Immunohistochemistry paraffin embedded sections - Anti-G3BP antibody [EPR13985(B)] (ab181149)

      Immunohistochemical analysis of paraffin-embedded Human muscle tissue staining G3BP using ab181149 at 1/100 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody with Hematoxylin counterstain.

    • Immunocytochemistry/ Immunofluorescence - Anti-G3BP antibody [EPR13985(B)] (ab181149)
      Immunocytochemistry/ Immunofluorescence - Anti-G3BP antibody [EPR13985(B)] (ab181149)

      Immunofluorescence analysis of 4% paraformaldehyde fixed 293 cells staining G3BP using ab181149 at 1/500 dilution (red), and Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution as a secondary antibody. Dapi was used for counterstaining (blue).

    • Flow Cytometry - Anti-G3BP antibody [EPR13985(B)] (ab181149)
      Flow Cytometry - Anti-G3BP antibody [EPR13985(B)] (ab181149)

      Flow cytometric analysis of 2% paraformaldehyde fixed Jurkat cells staining G3BP using ab181149 at 1/10 dilution, and FITC conjugated Goat anti rabbit IgG at 1/150 dilution as a secondary antibody (red curve). Rabbit monoclonal IgG was the negative control (green curve).

       

    • Immunoprecipitation - Anti-G3BP antibody [EPR13985(B)] (ab181149)
      Immunoprecipitation - Anti-G3BP antibody [EPR13985(B)] (ab181149)

      Western blot analysis of G3BP in immunoprecipitation pellets from Ramos cell lysate, using ab181149 at a 1/50 dilution. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG was used as a secondary antibody at 1/1000 dilution. Blocking/dilution buffer and concentration: 5% NFDM/TBST

    References

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