Overview

  • Product name
    Anti-G3BP antibody [EPR13986(B)]
    See all G3BP primary antibodies
  • Description
    Rabbit monoclonal [EPR13986(B)] to G3BP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human G3BP aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13283

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Hela, Jurkat, Ramos and 293 cell lysates; 293 cells; Human colon tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/50 - 1/100.
IP 1/20.

Target

  • Function
    May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 1 NTF2 domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Domain
    The NTF2 domain mediates multimerization.
  • Post-translational
    modifications
    Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
    Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytosol. Cell membrane. Nucleus. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ATP dependent DNA helicase VIII antibody
    • ATP-dependent DNA helicase VIII antibody
    • G3BP antibody
    • G3BP stress granule assembly factor 1 antibody
    • G3BP-1 antibody
    • G3bp1 antibody
    • G3BP1_HUMAN antibody
    • GAP binding protein antibody
    • GAP SH3 domain binding protein 1 antibody
    • GAP SH3 domain-binding protein 1 antibody
    • GTPase activating protein (SH3 domain) binding protein 1 antibody
    • hDH VIII antibody
    • Human DNA helicase VIII antibody
    • MGC111040 antibody
    • Ras GTPase activating protein binding protein 1 antibody
    • Ras GTPase activating protein SH3 domain binding protein antibody
    • Ras GTPase-activating protein-binding protein 1 antibody
    • RasGAP associated endoribonuclease G3BP antibody
    see all

Images

  • Western blot - Anti-G3BP antibody [EPR13986(B)] (ab181150)
    Western blot - Anti-G3BP antibody [EPR13986(B)] (ab181150)
    All lanes : Anti-G3BP antibody [EPR13986(B)] (ab181150) at 1/5000 dilution

    Lane 1 : Jurkat cell lysate with NFDM/TBST
    Lane 2 : Ramos cell lysate with NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 52 kDa
    Observed band size: 68 kDa (why is the actual band size different from the predicted?)

  • Western blot - Anti-G3BP antibody [EPR13986(B)] (ab181150)
    Western blot - Anti-G3BP antibody [EPR13986(B)] (ab181150)
    All lanes : Anti-G3BP antibody [EPR13986(B)] (ab181150) at 1/1000 dilution

    Lane 1 : Hela cell lysate with NFDM/TBST
    Lane 2 : 293 cell lysate with NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 52 kDa
    Observed band size: 68 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemistry paraffin embedded sections - Anti-G3BP antibody [EPR13986(B)] (ab181150)
    Immunohistochemistry paraffin embedded sections - Anti-G3BP antibody [EPR13986(B)] (ab181150)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue staining G3BP using ab181150 at 1/100 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody with Hematoxylin counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-G3BP antibody [EPR13986(B)] (ab181150)
    Immunocytochemistry/ Immunofluorescence - Anti-G3BP antibody [EPR13986(B)] (ab181150)

    Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells staining G3BP using ab181150 at 1/100 dilution, and Alexa Fluor®555 stained Goat anti Rabbit IgG at 1/200 dilution as a secondary antibody (red). Dapi counterstain (blue)

  • Immunoprecipitation - Anti-G3BP antibody [EPR13986(B)] (ab181150)
    Immunoprecipitation - Anti-G3BP antibody [EPR13986(B)] (ab181150)

    Western blot analysis of G3BP in immunoprecipitation pellets from Ramos cell lysate, using ab181151 at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as a secondary antibody at 1/1000 dilution. Blocking/dilution buffer and concentration: 5% NFDM/TBST



    Anti-G3BP antibody [EPR13986(B)] (ab181150) at 1/50 dilution + Immunoprecipitate from Ramos cell lysate using ab181150 with NFDM/TBST at 5 %

    Secondary
    Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

References

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