Anti-G3BP (phospho S232) antibody (ab131243)
Key features and details
- Rabbit polyclonal to G3BP (phospho S232)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-G3BP (phospho S232) antibody
See all G3BP primary antibodies -
Description
Rabbit polyclonal to G3BP (phospho S232) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Hela cells; Extracts from 293 cells treated with starvation; Human breast carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131243 was purified by affinity chromatography using peptide-specific phosphopeptide. Non-phosphosepcific antibodies were removed by chromatography using non-specific peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 52 kDa.
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 52 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
Target
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Function
May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 NTF2 domain.
Contains 1 RRM (RNA recognition motif) domain. -
Domain
The NTF2 domain mediates multimerization. -
Post-translational
modificationsPhosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
Arg-435 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Cytoplasm. Cytoplasm > cytosol. Cell membrane. Nucleus. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS. - Information by UniProt
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Database links
- Entrez Gene: 10146 Human
- Omim: 608431 Human
- SwissProt: Q13283 Human
- Unigene: 587054 Human
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Alternative names
- ATP dependent DNA helicase VIII antibody
- ATP-dependent DNA helicase VIII antibody
- G3BP antibody
see all
Images
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Immunofluorescence analysis of methanol-fixed Hela cells labelling G3BP (phospho S232) with ab131243 at 1/100 dilution.
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All lanes : Anti-G3BP (phospho S232) antibody (ab131243) at 1/500 dilution
Lane 1 : Untreated 293 cell extract
Lane 2 : Extract from 293 cells treated with starvation
Predicted band size: 52 kDa -
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling G3BP (phospho S232) with ab131243 at 1/50 dilution. Right panel was preincubated with blocking peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131243 has not yet been referenced specifically in any publications.