Key features and details
- Rabbit polyclonal to G3BP (phospho S232)
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-G3BP (phospho S232) antibody
See all G3BP primary antibodies
DescriptionRabbit polyclonal to G3BP (phospho S232)
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human G3BP (phospho S232). (Phospho specific peptide).
Database link: Q13283
- Starvation treated 293 cell extract; Human breast carcinoma, HeLa cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab194925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/2000. Predicted molecular weight: 52 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionMay be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
Sequence similaritiesContains 1 NTF2 domain.
Contains 1 RRM (RNA recognition motif) domain.
DomainThe NTF2 domain mediates multimerization.
modificationsPhosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
Cellular localizationCytoplasm. Cytoplasm > cytosol. Cell membrane. Nucleus. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.
- Information by UniProt
- ATP dependent DNA helicase VIII antibody
- ATP-dependent DNA helicase VIII antibody
- G3BP antibody
All lanes : Anti-G3BP (phospho S232) antibody (ab194925) at 1/500 dilution
Lane 1 : Untreated 293 cell extract
Lane 2 : Starvation treated 293 cell extract
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
ab194925 has not yet been referenced specifically in any publications.