Overview

  • Product name
  • Description
    Rabbit polyclonal to G3BP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 275 - 325 of Human G3BP2 (NP_036429.2)

  • Positive control
    • Whole cell lysate from HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Probable scaffold protein that may be involved in mRNA transport.
  • Sequence similarities
    Contains 1 NTF2 domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Arg-457 and Arg-468 are dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • G3BP 2 antibody
    • G3BP-2 antibody
    • G3BP2 antibody
    • G3BP2_HUMAN antibody
    • GAP SH3 domain binding protein 2 antibody
    • GAP SH3 domain-binding protein 2 antibody
    • GTPase activating protein (SH3 domain) binding protein 2 antibody
    • Ras GTPase activating protein SH3 domain binding protein 2 antibody
    • Ras GTPase-activating protein-binding protein 2 antibody
    see all

Images

  • All lanes : Anti-G3BP2 antibody (ab86135) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    Developed using the ECL technique.

    Predicted band size: 54 kDa


    Exposure time: 60 seconds
  • 1mg whole cell lysate from HeLa cells was immunoprecipitated using 3µg ab86135.
    20% of IP was loaded per lane, and probed with ab86135 at 1µg/ml (lane 1) or with a control IgG (lane 2).
    Detection: chemiluminescence with an exposure time of 30 seconds.
  • ICC/IF image of ab86135 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86135, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Liao KC  et al. Identification and characterization of host proteins bound to dengue virus 3' UTR reveal an antiviral role for quaking proteins. RNA 24:803-814 (2018). Read more (PubMed: 29572260) »
  • Gupta N  et al. Stress granule-associated protein G3BP2 regulates breast tumor initiation. Proc Natl Acad Sci U S A 114:1033-1038 (2017). Read more (PubMed: 28096337) »

See all 6 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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