Key features and details
- Rabbit polyclonal to G3BP2
- Suitable for: ICC/IF, WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-G3BP2 antibody
See all G3BP2 primary antibodies
DescriptionRabbit polyclonal to G3BP2
Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 275 - 325 of Human G3BP2 (NP_036429.2)
- Whole cell lysate from HeLa cells
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 54 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionProbable scaffold protein that may be involved in mRNA transport.
Sequence similaritiesContains 1 NTF2 domain.
Contains 1 RRM (RNA recognition motif) domain.
modificationsArg-457 and Arg-468 are dimethylated, probably to asymmetric dimethylarginine.
- Information by UniProt
- G3BP 2 antibody
- G3BP-2 antibody
- G3BP2 antibody
All lanes : Anti-G3BP2 antibody (ab86135) at 0.4 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 60 seconds
1mg whole cell lysate from HeLa cells was immunoprecipitated using 3µg ab86135.
20% of IP was loaded per lane, and probed with ab86135 at 1µg/ml (lane 1) or with a control IgG (lane 2). Detection: chemiluminescence with an exposure time of 30 seconds.
ICC/IF image of ab86135 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86135, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab86135 has been referenced in 11 publications.
- Lei Y et al. O-GlcNAcylation of PFKFB3 is required for tumor cell proliferation under hypoxia. Oncogenesis 9:21 (2020). PubMed: 32060258
- Pla-Martín D et al. CLUH granules coordinate translation of mitochondrial proteins with mTORC1 signaling and mitophagy. EMBO J 39:e102731 (2020). PubMed: 32149416
- Liao KC et al. Identification and characterization of host proteins bound to dengue virus 3' UTR reveal an antiviral role for quaking proteins. RNA 24:803-814 (2018). PubMed: 29572260
- Mao C et al. A G3BP1-Interacting lncRNA Promotes Ferroptosis and Apoptosis in Cancer via Nuclear Sequestration of p53. Cancer Res 78:3484-3496 (2018). PubMed: 29588351
- Hartmann H et al. Proteomics and C9orf72 neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity. Life Sci Alliance 1:e201800070 (2018). PubMed: 30456350