Product nameAnti-G3BP2 antibody
See all G3BP2 primary antibodies
DescriptionRabbit polyclonal to G3BP2
Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 275 - 325 of Human G3BP2 (NP_036429.2)
- Whole cell lysate from HeLa cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 54 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionProbable scaffold protein that may be involved in mRNA transport.
Sequence similaritiesContains 1 NTF2 domain.
Contains 1 RRM (RNA recognition motif) domain.
modificationsArg-457 and Arg-468 are dimethylated, probably to asymmetric dimethylarginine.
- Information by UniProt
- G3BP 2 antibody
- G3BP-2 antibody
- G3BP2 antibody
All lanes : Anti-G3BP2 antibody (ab86135) at 0.4 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 60 seconds
1mg whole cell lysate from HeLa cells was immunoprecipitated using 3µg ab86135.
20% of IP was loaded per lane, and probed with ab86135 at 1µg/ml (lane 1) or with a control IgG (lane 2). Detection: chemiluminescence with an exposure time of 30 seconds.
ICC/IF image of ab86135 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86135, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Liao KC et al. Identification and characterization of host proteins bound to dengue virus 3' UTR reveal an antiviral role for quaking proteins. RNA 24:803-814 (2018). Read more (PubMed: 29572260) »
- Mao C et al. A G3BP1-Interacting lncRNA Promotes Ferroptosis and Apoptosis in Cancer via Nuclear Sequestration of p53. Cancer Res 78:3484-3496 (2018). Read more (PubMed: 29588351) »