Product nameG5, Ubiquitin Isopeptidase Inhibitor I
- 3,5-bis((4-Nitrophenyl)methylene)-1,1-dioxide, tetrahydro-4H-thiopyran-4-one
Cell-permeable cross-conjugated α,β-unsaturated dienone compound reported to induce caspase activation and apoptosis (IC50 = 1.76 and 1.6 µM in E1A and E1A/C9DN cells respectively) via an apoptosome-independent mitochondrial pathway by selectively inhibiting ubiquitin isopeptidase activity. The cellular expression of Bax and Bak is essential for G5-activated apoptosis, while Bcl-2 appears to protect cells against the effect of G5.
Storage instructionsStore at +4°C. The product can be stored for up to 12 months.
Soluble in a 1:3 solution of DMSO:PBS. Please see Handling instructions.
For maximum solubility we recommend this compound be dissolved in DMSO and then diluted with aqueous buffer of choice. This compound has a solubility of approximately 0.25 mg/ml in a 1:3 solution of DMSO:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day. Also, DMSO solution needs to be purged with an inert gas for stability.
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This product has been referenced in:
- Fontanini A et al. The Isopeptidase Inhibitor G5 Triggers a Caspase-independent Necrotic Death in Cells Resistant to Apoptosis: A COMPARATIVE STUDY WITH THE PROTEASOME INHIBITOR BORTEZOMIB. J Biol Chem 284:8369-81 (2009). Read more (PubMed: 19139105) »
- Aleo E et al. Identification of new compounds that trigger apoptosome-independent caspase activation and apoptosis. Cancer Res 66:9235-44 (2006). Read more (PubMed: 16982768) »