Product nameAnti-G5PR antibody
See all G5PR primary antibodies
DescriptionRabbit polyclonal to G5PR
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human G5PR aa 1-160.
Database link: Q969Q6
- WB: Mouse stomach and brain tissue lysate; A431, Jurkat and A549 whole cell lysates. IHC-P: Human brain and gastric cancer tissues.
Previously labelled as PPP2R3C.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Mouse stomach normal tissue lysate - total protein (ab29256)
- Jurkat whole cell lysate (ab30128)
- A431 whole cell lysate (ab30132)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (ab4022)
- Jurkat whole cell lysate (ab7899)
- A431 whole cell lysate (ab7909)
- A549 whole cell lysate (ab7910)
Our Abpromise guarantee covers the use of ab230347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 53, 30 kDa (predicted molecular weight: 53, 40 kDa).|
FunctionMay regulate MCM3AP phosphorylation through phosphatase recruitment. May play a role in the activation-induced cell death of B-cells.
Tissue specificityUbiquitously expressed in brain and other tissues.
Sequence similaritiesContains 2 EF-hand domains.
Developmental stageExpressed in fetal brain.
Cellular localizationNucleus. Cytoplasm. Excluded from the nucleoli. Localization is cell cycle-dependent. Localizes to the cytoplasm during cytokinesis.
- Information by UniProt
- C14orf10 antibody
- Chromosome 14 open reading frame 10 antibody
- G4-1 antibody
All lanes : Anti-G5PR antibody (ab230347) at 1/1000 dilution
Lane 1 : Mouse stomach tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : A549 (human lung carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 53, 40 kDa
Observed band size: 30,53 kDa why is the actual band size different from the predicted?
Paraffin-embedded human brain tissue stained for G5PR with ab230347 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human gastric cancer tissue stained for G5PR with ab230347 at 1/100 dilution in immunohistochemical analysis.
ab230347 has not yet been referenced specifically in any publications.