Overview

  • Product name

  • Description

    Rabbit polyclonal to G5PR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human G5PR aa 1-160.
    Database link: Q969Q6

  • Positive control

    • WB: Mouse stomach and brain tissue lysate; A431, Jurkat and A549 whole cell lysates. IHC-P: Human brain and gastric cancer tissues.
  • General notes

    Previously labelled as PPP2R3C. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/1000 - 1/5000. Detects a band of approximately 53, 30 kDa (predicted molecular weight: 53, 40 kDa).

Target

  • Function

    May regulate MCM3AP phosphorylation through phosphatase recruitment. May play a role in the activation-induced cell death of B-cells.
  • Tissue specificity

    Ubiquitously expressed in brain and other tissues.
  • Sequence similarities

    Contains 2 EF-hand domains.
  • Developmental stage

    Expressed in fetal brain.
  • Cellular localization

    Nucleus. Cytoplasm. Excluded from the nucleoli. Localization is cell cycle-dependent. Localizes to the cytoplasm during cytokinesis.
  • Information by UniProt
  • Database links

  • Alternative names

    • C14orf10 antibody
    • Chromosome 14 open reading frame 10 antibody
    • G4-1 antibody
    • G5pr antibody
    • Hypothetical protein FLJ20644 antibody
    • P2R3C_HUMAN antibody
    • ppp2r3c antibody
    • protein phosphatase 2 (formerly 2A), regulatory subunit B'', gamma antibody
    • Protein phosphatase 2 regulatory subunit B''gamma antibody
    • protein phosphatase 2, regulatory subunit B'', gamma antibody
    • Protein phosphatase subunit G5PR antibody
    • rhabdomyosarcoma antigen MU-RMS-40.6A antibody
    • Rhabdomyosarcoma antigen MU-RMS-40.6A/6C antibody
    • rhabdomyosarcoma antigen Mu-RMS-40.6C antibody
    • serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma antibody
    see all

Images

  • All lanes : Anti-G5PR antibody (ab230347) at 1/1000 dilution

    Lane 1 : Mouse stomach tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 5 : A549 (human lung carcinoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 53, 40 kDa
    Observed band size: 30,53 kDa
    why is the actual band size different from the predicted?

  • Paraffin-embedded human brain tissue stained for G5PR with ab230347 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human gastric cancer tissue stained for G5PR with ab230347 at 1/100 dilution in immunohistochemical analysis.

References

ab230347 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab230347.
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