Key features and details
- Rabbit polyclonal to G5PR
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-G5PR antibody
See all G5PR primary antibodies
DescriptionRabbit polyclonal to G5PR
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human G5PR aa 1-160.
Database link: Q969Q6
- WB: Mouse stomach and brain tissue lysate; A431, Jurkat and A549 whole cell lysates. IHC-P: Human brain and gastric cancer tissues.
Previously labelled as PPP2R3C.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Mouse stomach normal tissue lysate - total protein (ab29256)
- Jurkat whole cell lysate (ab30128)
- A431 whole cell lysate (ab30132)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (ab4022)
- Jurkat whole cell lysate (ab7899)
- A431 whole cell lysate (ab7909)
- A549 whole cell lysate (ab7910)
Our Abpromise guarantee covers the use of ab230347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 53, 30 kDa (predicted molecular weight: 53, 40 kDa).|
FunctionMay regulate MCM3AP phosphorylation through phosphatase recruitment. May play a role in the activation-induced cell death of B-cells.
Tissue specificityUbiquitously expressed in brain and other tissues.
Sequence similaritiesContains 2 EF-hand domains.
Developmental stageExpressed in fetal brain.
Cellular localizationNucleus. Cytoplasm. Excluded from the nucleoli. Localization is cell cycle-dependent. Localizes to the cytoplasm during cytokinesis.
- Information by UniProt
- C14orf10 antibody
- Chromosome 14 open reading frame 10 antibody
- G4-1 antibody
All lanes : Anti-G5PR antibody (ab230347) at 1/1000 dilution
Lane 1 : Mouse stomach tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : A549 (human lung carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 53, 40 kDa
Observed band size: 30,53 kDa why is the actual band size different from the predicted?
Paraffin-embedded human brain tissue stained for G5PR with ab230347 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human gastric cancer tissue stained for G5PR with ab230347 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230347 has not yet been referenced specifically in any publications.