Overview

  • Product name

  • Description

    Rabbit polyclonal to G6PC3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 86-135 (ESGYYSQAPA QVHQFPSSCE TGPGSPSGHC MITGAALWPI MTALSSQVAT) of Human G6PC3 (NP_612396).

  • Positive control

    • 721_B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab133964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.

Target

  • Relevance

    G6PC3 is the catalytic subunit of glucose-6-phosphatase (G6Pase). G6Pase is located in the endoplasmic reticulum (ER) and catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the last step of the gluconeogenic and glycogenolytic pathways. Mutations in this gene result in autosomal recessive severe congenital neutropenia. Multiple transcript variants have been found for this gene, only one of which is expected to express a protein.
  • Cellular localization

    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • Database links

  • Alternative names

    • G-6-Pase 3 antibody
    • G6Pase 3 antibody
    • G6Pase-beta antibody
    • Glucose 6 phosphatase, catalytic, 3 antibody
    • Glucose-6-phosphatase 3 antibody
    • Glucose-6-phosphatase beta antibody
    • SCN4 antibody
    • Ubiquitous glucose-6-phosphatase catalytic subunit-related protein antibody
    • UGRP antibody
    see all

Images

  • Anti-G6PC3 antibody (ab133964) at 1 µg/ml + 721_B cell lysate at 10 µg

    Predicted band size: 39 kDa

References

This product has been referenced in:

  • Müller MS  et al. Effective Glucose Uptake by Human Astrocytes Requires Its Sequestration in the Endoplasmic Reticulum by Glucose-6-Phosphatase-ß. Curr Biol 28:3481-3486.e4 (2018). Read more (PubMed: 30415704) »
  • Tang Y  et al. Overexpression of PCK1 Gene Antagonizes Hepatocellular Carcinoma Through the Activation of Gluconeogenesis and Suppression of Glycolysis Pathways. Cell Physiol Biochem 47:344-355 (2018). Read more (PubMed: 29768256) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Sara McMillin

Verified customer

Submitted Jul 14 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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