Anti-G9P antibody (ab100996)
Key features and details
- Rabbit polyclonal to G9P
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-G9P antibody
See all G9P primary antibodies -
Description
Rabbit polyclonal to G9P -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Macaque monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human G9P aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab115936) -
Positive control
- This antibody gave a positive signal in Human spleen tissue lysate as well as the following whole cell lysates: MDA MB 231; MDA MB 361; HeLa.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab100996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa). |
Target
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Function
Receptor on natural killer (NK) cells for HLA-C alleles. Inhibits the activity of NK cells thus preventing cell lysis. -
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3805 Human
- Omim: 604945 Human
- SwissProt: Q99706 Human
- Unigene: 651287 Human
- Unigene: 661219 Human
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Alternative names
- CD_antigen=CD158d antibody
- CD158 antigen like family member D antibody
- CD158 antigen-like family member D antibody
see all
Images
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All lanes : Anti-G9P antibody (ab100996) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesThe band observed at 39 kDa could potentially be a cleaved form of G9P due to the presence of a 21 amino acid signal peptide.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab100996 has not yet been referenced specifically in any publications.