Key features and details
- Rabbit polyclonal to GAB1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-GAB1 antibody
See all GAB1 primary antibodies
DescriptionRabbit polyclonal to GAB1
Specificityab59362 detects endogenous levels of total GAB1 protein
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthesized non phosphopeptide derived from human GAB1 around the phosphorylation site of tyrosine 659 (VDYPVV).
General notesStore at -20°C for 1 year
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from rabbit antiserum by affinity chromatography, using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab59362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 77 kDa.|
|IHC-P||Use at an assay dependent concentration.|
FunctionProbably involved in EGF and insulin receptor signaling.
Sequence similaritiesBelongs to the GAB family.
Contains 1 PH domain.
modificationsPhosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.
- Information by UniProt
- Gab 1 antibody
- GAB1 antibody
- GAB1_HUMAN antibody
All lanes : Anti-GAB1 antibody (ab59362) at 1/500 dilution
Lane 1 : Extracts from NIH/3T3 cells with no immunizing peptide
Lane 2 : Extracts from NIH/3T3 cells with immunizing peptide
Observed band size: 77 kDa why is the actual band size different from the predicted?
ab59362, at a 1/50 dilution, staining human GAB1 in breast carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence of the immunizing peptide (left image) and in the presence of the immunizing peptide (right image).
ICC/IF image of ab59362 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59362, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab59362 has been referenced in 3 publications.
- Zhao T et al. DNA methylation-regulated QPCT promotes sunitinib resistance by increasing HRAS stability in renal cell carcinoma. Theranostics 9:6175-6190 (2019). PubMed: 31534544
- Hu L & Liu R Expression of Gab1 Is Associated with Poor Prognosis of Patients with Epithelial Ovarian Cancer. Tohoku J Exp Med 239:177-84 (2016). PubMed: 27302321
- Zhang Y et al. Identification of GRB2 and GAB1 coexpression as an unfavorable prognostic factor for hepatocellular carcinoma by a combination of expression profile and network analysis. PLoS One 8:e85170 (2013). IHC . PubMed: 24391994