Overview

  • Product name

    Anti-GAB1 antibody [EPR375]
    See all GAB1 primary antibodies
  • Description

    Rabbit monoclonal [EPR375] to GAB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GAB1 aa 500-600. The exact sequence is proprietary.
    Database link: Q13480

  • Positive control

    • WB: HepG2, K562, 293T, and SH-SY5Y cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Probably involved in EGF and insulin receptor signaling.
    • Sequence similarities

      Belongs to the GAB family.
      Contains 1 PH domain.
    • Post-translational
      modifications

      Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.
    • Information by UniProt
    • Database links

    • Alternative names

      • Gab 1 antibody
      • GAB1 antibody
      • GAB1_HUMAN antibody
      • GRB 2 associated binder 1 antibody
      • GRB 2 associated binding protein 1 antibody
      • GRB2 associated binding protein 1 isoform a antibody
      • GRB2 associated binding protein 1 isoform b antibody
      • GRB2-associated binder 1 antibody
      • GRB2-associated-binding protein 1 antibody
      • Growth factor receptor bound protein 2-associated protein 1 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: GAB1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: Hek293 whole cell lysate (20 µg)
      Lane 4: HepG2 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab133486 observed at 100 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab133486 was shown to recognize GAB1 in wild-type HAP1 cells as signal was lost at the expected MW in GAB1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GAB1 knockout samples were subjected to SDS-PAGE. Ab133486 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-GAB1 antibody [EPR375] (ab133486) at 1/2000 dilution (purified) + K562 cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 77 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-GAB1 antibody [EPR375] (ab133486) at 1/2000 dilution (purified) + HEK293 cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 77 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-GAB1 antibody [EPR375] (ab133486) at 1/1000 dilution (unpurified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 77 kDa

    • ab133486 (purified) at 1/80 immunoprecipitating GAB1 in HEK293 whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/10,000) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    References

    ab133486 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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