Overview

  • Product name

    Anti-GAB1 (phospho Y627) antibody
    See all GAB1 primary antibodies
  • Description

    Rabbit polyclonal to GAB1 (phospho Y627)
  • Host species

    Rabbit
  • Specificity

    ab131458 detects endogenous levels of GAB1 only when phosphorylated at Tyrosine 627.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from a region surrounding the phosphorylation site of Tyrosine 627 (

    V-E-Y(p)L-D

    ) of Human GAB1 (NP_002030.2).

  • Positive control

    • Methanol fixed HeLa cells; HUVEC cell extracts EGF treated.

Properties

Applications

Our Abpromise guarantee covers the use of ab131458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 77 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Probably involved in EGF and insulin receptor signaling.
  • Sequence similarities

    Belongs to the GAB family.
    Contains 1 PH domain.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.
  • Information by UniProt
  • Database links

  • Alternative names

    • Gab 1 antibody
    • GAB1 antibody
    • GAB1_HUMAN antibody
    • GRB 2 associated binder 1 antibody
    • GRB 2 associated binding protein 1 antibody
    • GRB2 associated binding protein 1 isoform a antibody
    • GRB2 associated binding protein 1 isoform b antibody
    • GRB2-associated binder 1 antibody
    • GRB2-associated-binding protein 1 antibody
    • Growth factor receptor bound protein 2-associated protein 1 antibody
    see all

Images

  • Immunofluorescence staining of methanol fixed HeLa cells labelling GAB1 (phospho Y627) with ab131458 antibody at a dilution of 1/100.
  • All lanes : Anti-GAB1 (phospho Y627) antibody (ab131458) at 1/500 dilution

    Lane 1 : HUVEC cell extracts untreated
    Lane 2 : HUVEC cell extracts EGF treated

    Predicted band size: 77 kDa

References

ab131458 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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