Anti-GAB2 antibody [EP369Y] (ab32365)


  • Product name
    Anti-GAB2 antibody [EP369Y]
    See all GAB2 primary antibodies
  • Description
    Rabbit monoclonal [EP369Y] to GAB2
  • Host species
  • Specificity
    This antibody is predicted to detect the splice isoform 2 based on sequence homology.
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GAB2 aa 600-700. The exact sequence is proprietary.

  • Positive control
    • PC12 cells; K562 cells; MCF7 cells
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab32365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 74 kDa).
ICC/IF 1/50.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC or IP.
  • Target

    • Function
      Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.
    • Sequence similarities
      Belongs to the GAB family.
      Contains 1 PH domain.
    • Domain
      The SH3-binding motifs mediate interaction with SHC1 and GRB2.
      The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.
    • Post-translational
      Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
      Dephosphorylated by PTPN11.
    • Cellular localization
      Cytoplasm. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • GAB 2 antibody
      • Gab2 antibody
      • GAB2_HUMAN antibody
      • Grb 2 associated binder 2 antibody
      • GRB 2 associated binding protein 2 antibody
      • Grb2 associated binder 2 antibody
      • GRB2 associated binder 2 pp100 antibody
      • GRB2 associated binding protein 2 antibody
      • GRB2-associated binder 2 antibody
      • GRB2-associated-binding protein 2 antibody
      • Growth factor receptor bound protein 2 associated protein 2 antibody
      • Growth factor receptor bound protein 2-associated protein 2 antibody
      • KIAA0571 antibody
      • p97 antibody
      • PH domain containing adaptor molecule p97 antibody
      • pp100 antibody
      see all


    • All lanes : Anti-GAB2 antibody [EP369Y] (ab32365) at 1/500 dilution

      Lane 1 : K526 cell lysate
      Lane 2 : MCF-7 cell lysate

      Predicted band size: 74 kDa
      Observed band size: 97 kDa (why is the actual band size different from the predicted?)

    • Immunofluorescent staining of PC-12 cells
      using ab32365 at 1/50 dilution


    This product has been referenced in:
    • Tian LQ  et al. MicroRNA-197 inhibits cell proliferation by targeting GAB2 in glioblastoma. Mol Med Rep N/A:N/A (2016). WB . Read more (PubMed: 27035789) »
    • Pyarajan S  et al. Interleukin-3 (IL-3)-induced c-fos Activation Is Modulated by Gab2-Calcineurin Interaction. J Biol Chem 283:23505-9 (2008). WB . Read more (PubMed: 18586679) »

    See all 2 Publications for this product

    Customer reviews and Q&As

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    Hello! The blocking peptides for these antibodies have now been published in our catalog, they are ab133951 and ab133952. Please let me know if you have any further questions.


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