Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP369Y] to GAB2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-GAB2 antibody [EP369Y]
See all GAB2 primary antibodies
DescriptionRabbit monoclonal [EP369Y] to GAB2
SpecificityThis antibody is predicted to detect the splice isoform 2 based on sequence homology.
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF,IHC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GAB2 aa 600-700. The exact sequence is proprietary.
- WB: K562 cell lysates
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
PurityWhole Cell Lysate
Our Abpromise guarantee covers the use of ab32365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 74 kDa).|
FunctionAdapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.
Sequence similaritiesBelongs to the GAB family.
Contains 1 PH domain.
DomainThe SH3-binding motifs mediate interaction with SHC1 and GRB2.
The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.
modificationsPhosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
Dephosphorylated by PTPN11.
Cellular localizationCytoplasm. Cell membrane.
- Information by UniProt
- GAB 2 antibody
- Gab2 antibody
- GAB2_HUMAN antibody
All lanes : Anti-GAB2 antibody [EP369Y] (ab32365) at 1/500 dilution
Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) prepared in RIPA lysis method
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) prepared in 1%SDS Hot lysis method
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?
We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
Blocking/Diluting Buffer and concentration:
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab32365 has been referenced in 2 publications.
- Tian LQ et al. MicroRNA-197 inhibits cell proliferation by targeting GAB2 in glioblastoma. Mol Med Rep N/A:N/A (2016). WB . PubMed: 27035789
- Pyarajan S et al. Interleukin-3 (IL-3)-induced c-fos Activation Is Modulated by Gab2-Calcineurin Interaction. J Biol Chem 283:23505-9 (2008). WB . PubMed: 18586679