Product nameAnti-GAB2 antibody [EP369Y]
See all GAB2 primary antibodies
DescriptionRabbit monoclonal [EP369Y] to GAB2
SpecificityThis antibody is predicted to detect the splice isoform 2 based on sequence homology.
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GAB2 aa 600-700. The exact sequence is proprietary.
- PC12 cells; K562 cells; MCF7 cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
PurityWhole Cell Lysate
Our Abpromise guarantee covers the use of ab32365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 74 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionAdapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.
Sequence similaritiesBelongs to the GAB family.
Contains 1 PH domain.
DomainThe SH3-binding motifs mediate interaction with SHC1 and GRB2.
The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.
modificationsPhosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
Dephosphorylated by PTPN11.
Cellular localizationCytoplasm. Cell membrane.
- Information by UniProt
- GAB 2 antibody
- Gab2 antibody
- GAB2_HUMAN antibody
All lanes : Anti-GAB2 antibody [EP369Y] (ab32365) at 1/500 dilution
Lane 1 : K526 cell lysate
Lane 2 : MCF-7 cell lysate
Predicted band size: 74 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?
Immunofluorescent staining of PC-12 cells
using ab32365 at 1/50 dilution
This product has been referenced in:
- Tian LQ et al. MicroRNA-197 inhibits cell proliferation by targeting GAB2 in glioblastoma. Mol Med Rep N/A:N/A (2016). WB . Read more (PubMed: 27035789) »
- Pyarajan S et al. Interleukin-3 (IL-3)-induced c-fos Activation Is Modulated by Gab2-Calcineurin Interaction. J Biol Chem 283:23505-9 (2008). WB . Read more (PubMed: 18586679) »