Product nameAnti-GAB2 (phospho Y452) antibody
See all GAB2 primary antibodies
DescriptionRabbit polyclonal to GAB2 (phospho Y452)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- Rat intestine and mouse embryo lysates; Human lung tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.01% BSA, 50% Glycerol
Aqueous buffered solution
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.
(or 1/50 - 1/200 if using a fluorescent secondary antibody)
|WB||1/100 - 1/1000. Predicted molecular weight: 74 kDa.|
FunctionAdapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.
Sequence similaritiesBelongs to the GAB family.
Contains 1 PH domain.
DomainThe SH3-binding motifs mediate interaction with SHC1 and GRB2.
The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.
modificationsPhosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
Dephosphorylated by PTPN11.
Cellular localizationCytoplasm. Cell membrane.
- Information by UniProt
- GAB 2 antibody
- Gab2 antibody
- GAB2_HUMAN antibody
All lanes : Anti-GAB2 (phospho Y452) antibody (ab203616) at 1/200 dilution
Lane 1 : Rat intestine lysate
Lane 2 : Mouse embryo lysate
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution
Predicted band size: 74 kDa
Observed band size: 80, 85 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung tissue labeling GAB2 with ab203616 at 1/200, followed by conjugation to the secondary antibody and DAB staining.
ab203616 has not yet been referenced specifically in any publications.