Key features and details
- Rabbit polyclonal to GAB2 (phospho Y643)
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GAB2 (phospho Y643) antibody
See all GAB2 primary antibodies
DescriptionRabbit polyclonal to GAB2 (phospho Y643)
SpecificityThis antibody detects endogenous levels of GAB2 only when phosphorylated at tyrosine 643.
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthesized phosphopeptide derived from human GAB2 around the phosphorylation site of tyrosine 643 (V-D-YP-V-Q).
- Extracts from Jurkat cells. Human brain tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab78262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionAdapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.
Sequence similaritiesBelongs to the GAB family.
Contains 1 PH domain.
DomainThe SH3-binding motifs mediate interaction with SHC1 and GRB2.
The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.
modificationsPhosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
Dephosphorylated by PTPN11.
Cellular localizationCytoplasm. Cell membrane.
- Information by UniProt
- GAB 2 antibody
- Gab2 antibody
- GAB2_HUMAN antibody
All lanes : Anti-GAB2 (phospho Y643) antibody (ab78262) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells, treated
with IFN (2500U/ML, 30mins)
Lane 2 : Extracts from Jurkat cells, treated
with IFN (2500U/ML, 30mins) plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size: 74 kDa
Observed band size: 74 kDa
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab78262 at a 1/50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
ab78262 has been referenced in 1 publication.
- Yim JH et al. Phosphoprotein profiles of candidate markers for early cellular responses to low-dose ?-radiation in normal human fibroblast cells. J Radiat Res 58:329-340 (2017). WB ; Human . PubMed: 28122968