Anti-GAB3 antibody (ab121311)

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Western blot - Anti-GAB3 antibody (ab121311)
  • Immunocytochemistry/ Immunofluorescence - Anti-GAB3 antibody (ab121311)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAB3 antibody (ab121311)

Key features and details

  • Rabbit polyclonal to GAB3
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-GAB3 antibody
    See all GAB3 primary antibodies

  • Description

    Rabbit polyclonal to GAB3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    antigen sequence, corresponding to amino acids 336-480 of Human GAB3.

  • Positive control

    • Human Plasma, Liver and Tonsil lysates; Human liver tissue

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.04 - 0.4 µg/ml.

Target

  • Sequence similarities

    Belongs to the GAB family.
    Contains 1 PH domain.

  • Post-translational
    modifications

    Phosphorylated on tyrosine residue(s) after macrophage colony-stimulating factor (M-CSF) receptor stimulation.
  • Information by UniProt

  • Database links

    • Alternative names

      • Gab3 antibody
      • GAB3_HUMAN antibody
      • GRB2-associated binder 3 antibody
      • GRB2-associated-binding protein 3 antibody
      • Growth factor receptor bound protein 2-associated protein 3 antibody
      see all

    Images

    • Western blot - Anti-GAB3 antibody (ab121311)
      Western blot - Anti-GAB3 antibody (ab121311)
      All lanes : Anti-GAB3 antibody (ab121311) at 1/250 dilution

      Lane 1 : RT4 cell lysate
      Lane 2 : U-251 MG cell lysate
      Lane 3 : Human Plasma
      Lane 4 : Human Liver lysate
      Lane 5 : Human Tonsil lysate

      Developed using the ECL technique.
    • Immunocytochemistry/ Immunofluorescence - Anti-GAB3 antibody (ab121311)
      Immunocytochemistry/ Immunofluorescence - Anti-GAB3 antibody (ab121311)
      Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab121311 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAB3 antibody (ab121311)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAB3 antibody (ab121311)
      ab121311 at 1/400 dilution staining GAB3 in Paraffin-embedded Human liver tissue by Immunohistochemistry.

    References (1)

    Publishing research using ab121311? Please let us know so that we can cite the reference in this datasheet.

    ab121311 has been referenced in 1 publication.

    • Wieczfinska J  et al. The whole-genome expression analysis of peripheral blood mononuclear cells from aspirin sensitive asthmatics versus aspirin tolerant patients and healthy donors after in vitro aspirin challenge. Respir Res 16:147 (2015). WB . PubMed: 26646719

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