Anti-GAB3 antibody (ab121311)

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Overview

  • Product name

    Anti-GAB3 antibody
    See all GAB3 primary antibodies

  • Description

    Rabbit polyclonal to GAB3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    antigen sequence, corresponding to amino acids 336-480 of Human GAB3.

  • Positive control

    • Human Plasma, Liver and Tonsil lysates; Human liver tissue

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Sequence similarities

    Belongs to the GAB family.
    Contains 1 PH domain.

  • Post-translational
    modifications

    Phosphorylated on tyrosine residue(s) after macrophage colony-stimulating factor (M-CSF) receptor stimulation.
  • Information by UniProt

  • Database links

    • Alternative names

      • Gab3 antibody
      • GAB3_HUMAN antibody
      • GRB2-associated binder 3 antibody
      • GRB2-associated-binding protein 3 antibody
      • Growth factor receptor bound protein 2-associated protein 3 antibody
      see all

    Images

    • Western blot - Anti-GAB3 antibody (ab121311)
      Western blot - Anti-GAB3 antibody (ab121311)
      All lanes : Anti-GAB3 antibody (ab121311) at 1/250 dilution

      Lane 1 : RT4 cell lysate
      Lane 2 : U-251 MG cell lysate
      Lane 3 : Human Plasma
      Lane 4 : Human Liver lysate
      Lane 5 : Human Tonsil lysate

      Developed using the ECL technique.
    • Immunocytochemistry/ Immunofluorescence - Anti-GAB3 antibody (ab121311)
      Immunocytochemistry/ Immunofluorescence - Anti-GAB3 antibody (ab121311)
      Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab121311 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAB3 antibody (ab121311)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAB3 antibody (ab121311)
      ab121311 at 1/400 dilution staining GAB3 in Paraffin-embedded Human liver tissue by Immunohistochemistry.

    References

    This product has been referenced in:

    • Wieczfinska J  et al. The whole-genome expression analysis of peripheral blood mononuclear cells from aspirin sensitive asthmatics versus aspirin tolerant patients and healthy donors after in vitro aspirin challenge. Respir Res 16:147 (2015). WB . Read more (PubMed: 26646719) »
    See 1 Publication for this product

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