Key features and details
- Rabbit polyclonal to GAB3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-GAB3 antibody
See all GAB3 primary antibodies
DescriptionRabbit polyclonal to GAB3
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment (His-T7-tag) corresponding to Mouse GAB3 aa 142-375. Two N-terminal tags. (Expressed in E.coli).
PASSLHTVHVANSALLKDDRNTNSVVTEETRRESEFLFLPDYLILSNCET GRLHHASLPTRCDSWSNSNHSLAQTSFDDVFLDGLQPFISNNLVHPLHHG KVSQDFPSIRPQASLIWNREINGPSRNLMSSSPLLESSLNPTVHVEEKQV SLPSGVKELNIMSNTPPPRPPKPSYLSEQRQDQPLLTGHSSNKKPGYTMV PRRISLSGLDHVGSWKGDVQSQSLRHRDKRLSLN
Database link: Q8BSM5
- IHC-P: Mouse liver, ovary, pancreas, heart and uterus tissues. WB: MCF7 and HepG2 cell lysate; Recombinant mouse GAB3 protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab233411 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 68 kDa.|
Sequence similaritiesBelongs to the GAB family.
Contains 1 PH domain.
modificationsPhosphorylated on tyrosine residue(s) after macrophage colony-stimulating factor (M-CSF) receptor stimulation.
- Information by UniProt
- Gab3 antibody
- GAB3_HUMAN antibody
- GRB2-associated binder 3 antibody
Paraffin-embedded mouse liver tissue stained for GAB3 using ab233411 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded mouse ovary tissue stained for GAB3 using ab233411 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded mouse pancreas tissue stained for GAB3 using ab233411 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded mouse heart tissue stained for GAB3 using ab233411 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded mouse uterus tissue stained for GAB3 using ab233411 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-GAB3 antibody (ab233411) at 5 µg/ml + Recombinant mouse GAB3 protein
Predicted band size: 68 kDa
All lanes : Anti-GAB3 antibody (ab233411) at 3 µg/ml
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 68 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233411 has not yet been referenced specifically in any publications.