Anti-GABA A Receptor alpha 1 antibody (ab33299)
Key features and details
- Rabbit polyclonal to GABA A Receptor alpha 1
- Suitable for: IHC-P, WB, IP
- Reacts with: Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GABA A Receptor alpha 1 antibody
See all GABA A Receptor alpha 1 primary antibodies -
Description
Rabbit polyclonal to GABA A Receptor alpha 1 -
Host species
Rabbit -
Specificity
This antibody is specific for the GABA A Receptor a1 subunit. -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Cow, Cynomolgus monkey
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Immunogen
Synthetic peptide:
QPSQDELKDNTTVFT-C
, corresponding to N terminal amino acids 1-15 of the mature Rat GABA A Receptor alpha 1 containing a cysteine at the C terminus for linkage to a carrier protein. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
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| WB | (1) |
1/10000. Predicted molecular weight: 52 kDa.
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| IP |
Use at an assay dependent concentration. 5ul immunoprecipitates the GABA A Receptor a1 subunit in rat brain microsomal preparation RIPA lysate.
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. |
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WB
1/10000. Predicted molecular weight: 52 kDa. |
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IP
Use at an assay dependent concentration. 5ul immunoprecipitates the GABA A Receptor a1 subunit in rat brain microsomal preparation RIPA lysate. |
Target
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Function
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. -
Involvement in disease
Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 29705 Rat
- SwissProt: P08219 Cow
- SwissProt: P62813 Rat
- Unigene: 28463 Rat
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Alternative names
- ECA4 antibody
- EIEE19 antibody
- EJM antibody
see all
Images
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Western blot - Anti-GABA A Receptor alpha 1 antibody (ab33299)Anti-GABA A Receptor alpha 1 antibody (ab33299) at 1/10000 dilution + rat brain microsomal preparation tissue lysate at 10 µg
Predicted band size: 52 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody (ab33299)Immunohistochemistry analysis of formalin fixed paraffin embedded rat hippocampus tissue prepared using heat-induced epitope retrieval (HIER) labeling GABA A Receptor alpha 1 using ab33299 at 1/500 dilution. Secondary antibody was an anti-rabbit antibody and HRP-DAB.
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Immunoprecipitation - Anti-GABA A Receptor alpha 1 antibody (ab33299)Immunoprecipitation analysis: 1 mg of RIPA lysate of rat brain microsomes
was immunoprecipitated with 5 uL of ab33299. Arrow indicates GABAA
Receptor a1 (~50kD).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (34)
ab33299 has been referenced in 34 publications.
- Du T et al. Deep brain stimulation of the anterior nuclei of the thalamus relieves basal ganglia dysfunction in monkeys with temporal lobe epilepsy. CNS Neurosci Ther 27:341-351 (2021). PubMed: 33085171
- Park HY et al. Humulus japonicus rescues autistic-like behaviours in the BTBR T+ Itpr3tf/J mouse model of autism. Mol Med Rep 23:N/A (2021). PubMed: 33880583
- Rudolph S et al. Cerebellum-Specific Deletion of the GABAA Receptor d Subunit Leads to Sex-Specific Disruption of Behavior. Cell Rep 33:108338 (2020). PubMed: 33147470
- Scott H et al. Maternal antioxidant treatment prevents the adverse effects of prenatal stress on the offspring's brain and behavior. Neurobiol Stress 13:100281 (2020). PubMed: 33344732
- Li C et al. Downregulation of microRNA-29c reduces pain after child delivery by activating the oxytocin-GABA pathway. Mol Med Rep 22:1921-1931 (2020). PubMed: 32705167