Anti-GABA A Receptor alpha 1 antibody (ab33299)
Key features and details
- Rabbit polyclonal to GABA A Receptor alpha 1
- Suitable for: IHC-P, WB, IP
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-GABA A Receptor alpha 1 antibody
See all GABA A Receptor alpha 1 primary antibodies -
Description
Rabbit polyclonal to GABA A Receptor alpha 1 -
Host species
Rabbit -
Specificity
This antibody is specific for the GABA A Receptor a1 subunit. -
Tested Applications & Species
Application Species IHC-P RatIP RatWB Rat -
Immunogen
Synthetic peptide:
QPSQDELKDNTTVFT-C
, corresponding to N terminal amino acids 1-15 of the mature Rat GABA A Receptor alpha 1 containing a cysteine at the C terminus for linkage to a carrier protein. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
IHC-P |
Rat
|
IP |
Rat
|
WB |
Rat
|
All applications |
Cow
Cynomolgus monkey
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Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
|
|
WB | (1) |
1/10000. Predicted molecular weight: 52 kDa.
|
IP |
Use at an assay dependent concentration. 5ul immunoprecipitates the GABA A Receptor a1 subunit in rat brain microsomal preparation RIPA lysate.
|
Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
1/10000. Predicted molecular weight: 52 kDa. |
IP
Use at an assay dependent concentration. 5ul immunoprecipitates the GABA A Receptor a1 subunit in rat brain microsomal preparation RIPA lysate. |
Target
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Function
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. -
Involvement in disease
Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 29705 Rat
- SwissProt: P08219 Cow
- SwissProt: P62813 Rat
- Unigene: 28463 Rat
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Alternative names
- ECA4 antibody
- EIEE19 antibody
- EJM antibody
see all
Images
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Anti-GABA A Receptor alpha 1 antibody (ab33299) at 1/10000 dilution + rat brain microsomal preparation tissue lysate at 10 µg
Predicted band size: 52 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody (ab33299)
Immunohistochemistry analysis of formalin fixed paraffin embedded rat hippocampus tissue prepared using heat-induced epitope retrieval (HIER) labeling GABA A Receptor alpha 1 using ab33299 at 1/500 dilution. Secondary antibody was an anti-rabbit antibody and HRP-DAB.
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Immunoprecipitation analysis: 1 mg of RIPA lysate of rat brain microsomes
was immunoprecipitated with 5 uL of ab33299. Arrow indicates GABAA
Receptor a1 (~50kD).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab33299 has been referenced in 22 publications.
- Shang Y et al. Over-expressed MST1 impaired spatial memory via disturbing neural oscillation patterns in mice. Genes Brain Behav N/A:e12678 (2020). PubMed: 32468668
- Kurata S et al. Capacitation of mouse sperm is modulated by gamma-aminobutyric acid (GABA) concentration. J Reprod Dev 65:327-334 (2019). PubMed: 31178551
- Hudry E et al. Opposing Roles of apolipoprotein E in aging and neurodegeneration. Life Sci Alliance 2:N/A (2019). PubMed: 30760557
- Mudd DB et al. TrkB Activation During a Critical Period Mimics the Protective Effects of Early Visual Experience on Perception and the Stability of Receptive Fields in Adult Superior Colliculus. J Neurosci N/A:N/A (2019). PubMed: 30940716
- Sears SMS et al. Decreased epileptogenesis in mice lacking the System xc- transporter occurs in association with a reduction in AMPA receptor subunit GluA1. Epilepsia Open 4:133-143 (2019). PubMed: 30868123