Product nameAnti-GABA A Receptor alpha 1 antibody
See all GABA A Receptor alpha 1 primary antibodies
DescriptionRabbit polyclonal to GABA A Receptor alpha 1
SpecificityThis antibody is specific for the GABA A Receptor a1 subunit.
Tested applicationsSuitable for: IHC-P, WB, IP, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human
Predicted to work with: Cow, Cynomolgus monkey
- WB and IP: Rat brain microsomal tissue extract
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab33299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500. PubMed: 20650017|
|WB||1/10000. Predicted molecular weight: 52 kDa.|
|IP||Use at an assay dependent concentration. 5ul immunoprecipitates the GABA A Receptor a1 subunit in rat brain microsomal preparation RIPA lysate.|
|IHC-Fr||Use at an assay dependent concentration. Note: use 4% para glutaraldehyde fixed frozen sections permeabilized with Triton X100.|
FunctionGABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Involvement in diseaseDefects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue.
Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.
Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
- Information by UniProt
- ECA4 antibody
- EIEE19 antibody
- EJM antibody
Anti-GABA A Receptor alpha 1 antibody (ab33299) at 1/10000 dilution + rat brain microsomal preparation tissue lysate at 10 µg
Predicted band size: 52 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.
Immunohistochemistry analysis of formalin fixed paraffin embedded rat hippocampus tissue prepared using heat-induced epitope retrieval (HIER) labeling GABA A Receptor alpha 1 using ab33299 at 1/500 dilution. Secondary antibody was an anti-rabbit antibody and HRP-DAB.
Immunoprecipitation analysis: 1 mg of RIPA lysate of rat brain microsomes
was immunoprecipitated with 5 uL of ab33299. Arrow indicates GABAA
Receptor a1 (~50kD).
ab33299 staining GABA A Receptor alpha 1 in human retinal tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed with 4% paraformaldehyde, blocked with 10% donkey serum for 20 minutes at 24°C, then incubated with ab33299 at a 1/100 dilution for 16 hours at 4°C. The secondary antibody used was an Alexa-Fluor 488 (green) conjugated donkey anti-rabbit polyclonal, used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).
IPL - inner plexiform layerINL - inner nuclear layer
OPL - outer plexiform layerONL - outer nuclear layer
INS - inner nuclear segments
This product has been referenced in:
- Hudry E et al. Opposing Roles of apolipoprotein E in aging and neurodegeneration. Life Sci Alliance 2:N/A (2019). Read more (PubMed: 30760557) »
- Mudd DB et al. TrkB Activation During a Critical Period Mimics the Protective Effects of Early Visual Experience on Perception and the Stability of Receptive Fields in Adult Superior Colliculus. J Neurosci N/A:N/A (2019). Read more (PubMed: 30940716) »