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Neuroscience Neurotransmitter Amino Acids GABA
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Anti-GABA A Receptor alpha 1 antibody (ab33299)

  • Datasheet
  • SDS
Reviews (3)Q&A (4)References (22)

Product price, shipping and contact information

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Western blot - Anti-GABA A Receptor alpha 1 antibody (ab33299)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody (ab33299)
  • Immunoprecipitation - Anti-GABA A Receptor alpha 1 antibody (ab33299)

Key features and details

  • Rabbit polyclonal to GABA A Receptor alpha 1
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Rat
  • Isotype: IgG

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Overview

  • Product name

    Anti-GABA A Receptor alpha 1 antibody
    See all GABA A Receptor alpha 1 primary antibodies
  • Description

    Rabbit polyclonal to GABA A Receptor alpha 1
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for the GABA A Receptor a1 subunit.
  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    IP
    Rat
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide:

    QPSQDELKDNTTVFT-C

    , corresponding to N terminal amino acids 1-15 of the mature Rat GABA A Receptor alpha 1 containing a cysteine at the C terminus for linkage to a carrier protein.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • GABA
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • GABA Receptors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human GABA A Receptor alpha 1 protein (ab131966)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab33299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Rat
IP
Rat
WB
Rat
All applications
Cow
Cynomolgus monkey
Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
WB (1)
1/10000. Predicted molecular weight: 52 kDa.
IP
Use at an assay dependent concentration. 5ul immunoprecipitates the GABA A Receptor a1 subunit in rat brain microsomal preparation RIPA lysate.
Notes
IHC-P
Use at an assay dependent concentration.
WB
1/10000. Predicted molecular weight: 52 kDa.
IP
Use at an assay dependent concentration. 5ul immunoprecipitates the GABA A Receptor a1 subunit in rat brain microsomal preparation RIPA lysate.

Target

  • Function

    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
  • Involvement in disease

    Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
    Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue.
  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.
  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Target information above from: UniProt accession P14867 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 29705 Rat
    • SwissProt: P08219 Cow
    • SwissProt: P62813 Rat
    • Unigene: 28463 Rat
    • Alternative names

      • ECA4 antibody
      • EIEE19 antibody
      • EJM antibody
      • EJM5 antibody
      • Gaba receptor alpha 1 polypeptide antibody
      • GABA(A) receptor antibody
      • GABA(A) receptor subunit alpha 1 antibody
      • GABA(A) receptor subunit alpha-1 antibody
      • GABA(A) receptor, alpha 1 antibody
      • GABRA 1 antibody
      • GABRA1 antibody
      • Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody
      • Gamma aminobutyric acid A receptor alpha 1 antibody
      • Gamma aminobutyric acid receptor subunit alpha 1 antibody
      • Gamma aminobutyric acid type A receptor alpha1 subunit antibody
      • Gamma-aminobutyric acid receptor subunit alpha-1 antibody
      • GBRA1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-GABA A Receptor alpha 1 antibody (ab33299)
      Western blot - Anti-GABA A Receptor alpha 1 antibody (ab33299)
      Anti-GABA A Receptor alpha 1 antibody (ab33299) at 1/10000 dilution + rat brain microsomal preparation tissue lysate at 10 µg

      Predicted band size: 52 kDa
      Observed band size: 51 kDa why is the actual band size different from the predicted?



      Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody (ab33299)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody (ab33299)

      Immunohistochemistry analysis of formalin fixed paraffin embedded rat hippocampus tissue prepared using heat-induced epitope retrieval (HIER) labeling GABA A Receptor alpha 1 using ab33299 at 1/500 dilution. Secondary antibody was an anti-rabbit antibody and HRP-DAB.

    • Immunoprecipitation - Anti-GABA A Receptor alpha 1 antibody (ab33299)
      Immunoprecipitation - Anti-GABA A Receptor alpha 1 antibody (ab33299)

      Immunoprecipitation analysis: 1 mg of RIPA lysate of rat brain microsomes
      was immunoprecipitated with 5 uL of ab33299. Arrow indicates GABAA
      Receptor a1 (~50kD).

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (22)

    Publishing research using ab33299? Please let us know so that we can cite the reference in this datasheet.

    ab33299 has been referenced in 22 publications.

    • Shang Y  et al. Over-expressed MST1 impaired spatial memory via disturbing neural oscillation patterns in mice. Genes Brain Behav N/A:e12678 (2020). PubMed: 32468668
    • Kurata S  et al. Capacitation of mouse sperm is modulated by gamma-aminobutyric acid (GABA) concentration. J Reprod Dev 65:327-334 (2019). PubMed: 31178551
    • Hudry E  et al. Opposing Roles of apolipoprotein E in aging and neurodegeneration. Life Sci Alliance 2:N/A (2019). PubMed: 30760557
    • Mudd DB  et al. TrkB Activation During a Critical Period Mimics the Protective Effects of Early Visual Experience on Perception and the Stability of Receptive Fields in Adult Superior Colliculus. J Neurosci N/A:N/A (2019). PubMed: 30940716
    • Sears SMS  et al. Decreased epileptogenesis in mice lacking the System xc- transporter occurs in association with a reduction in AMPA receptor subunit GluA1. Epilepsia Open 4:133-143 (2019). PubMed: 30868123
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GABA A Receptor alpha 1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human Cell (SH-SY5Y))
    Permeabilization
    Yes - Triton X-100 (0'25%)
    Specification
    Human Cell (SH-SY5Y)
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 30 2016

    Western blot abreview for Anti-GABA A Receptor alpha 1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Cortical)
    Loading amount
    20 µg
    Specification
    Cortical
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Dr. Martin Broadstock

    Verified customer

    Submitted Aug 16 2012

    Question

    anti-GABA A
    Guide anticorps secondaires

    Read More

    Abcam community

    Verified customer

    Asked on Aug 07 2012

    Answer

    Merci de nous avoir contactés.
    Voici les liens vers les guides et posters qui je l'espère vous seront utiles:
    > DyLight® conjugated secondary antibodies : https://docs.abcam.com/pdf/general/DyLight_flyer.pdf
    > A Guide to Fluorochromes : https://docs.abcam.com/pdf/immunology/fluorochrome_guide.pdf
    > How to choose the right secondary antibody : https://www.abcam.com/index.html?pageconfig=resource&rid=12825
    > How to choose a conjugate : https://www.abcam.com/index.html?pageconfig=resource&rid=12922
    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Read More

    Abcam Scientific Support

    Answered on Aug 07 2012

    Question

    Hi Tech Support, how are you?

    Would you be able to help us with the following enquiry please:

    Customer’s question:

    I have some questions for you regarding the above antibody.

    I am working with guinea pig protein brain samples. I used Invitrogen PARIS kit that allows RNA and protein isolation from the same sample simultaneously. In this kit, after adding the lysis buffer and homogenising the brain sample, the homogenised sample can be immediately be kept aside without any further action for protein analysis. I am using B-actin as reference for my western blot analysis. I am trying to measure a lot of samples (control vs treated vs time-points) and I am then using ImageJ to analyse the densitometry of my bands relative to B-actin to get a relative protein expression value.

    For my western blot protocol this is what I do:

    Measure protein conc. using BCA kit (high conc.)

    Use 15ug protein per sample (dilute with water and 6x loading dye added). Load a total of 12uL.

    Heat at 95 degrees for 5 mins

    Run on Biorad TGX pre-made gel at 300V for 20mins

    Transfer using Biorad TGX transfer system for 5mins

    Ponceau S for 5 mins (all bands good transfer)

    Block 1 hr in 5% skim milk in TBST

    Primary ab overnight in blocking buffer (1:800 for gaba a antibody) at 4 degrees in dark cold room

    Next day, wash 3x for 5 mins in TBST

    Sec ab (anti rabbit, 1:10,000) for 1 hr at room temp in blocking buffer

    Wash 3x for 5 mins in TBST

    Use ECL for detection (from Milipore)

    Detect bands using Biorad ECL transilluminator

    Previously, I have combined the antibodies of GABA A and B-actin in the same solution for primary antibody incubation and use anti-mouse and anti-rabbit in the same secondary antibody incubation on the blot (detecting two proteins at the same time on the same blot). From these results, I got two very distinct bands, one at the ˜48kDa area (which I thought is the B-actin band) and a very distinct one at ˜75kDa (which I assumed is the GABA-A band).

    Of course, looking at the data sheet, this did not make sense as GABA-A is 52-55kDa. So I did not understand why I was seeing a very distinct band at the 75kDa area. I repeated this many many times and for many many samples and kept getting the same results, though each time when I quantified the intensity of the assumed GABA-A bands, it changed each time giving me highly variable results.

    So then, I separated out the antibodies and did them individually. After Ponceau S staining, I cut the membrane up one 50kDa and below, and the other is the top half (containing the 75kDa mark). I made different primary ab solutions and incubated them separately. B-actin came out perfectly but there was nothing for the top half of the membrane (with GABA-A antibody).

    I then repeated the whole thing again from the start, using the same protein samples except this time, I did purely GABA-A antibody alone without cutting the membrane up at all. Nothing came up, not even at the 52-55kDa area.

    Now of course I am stuck. I reordered a new antibody (ab33299) but I am now considering trying from a different company or getting a positive sample in like HeLa or HepG2 cell line to test if the GABA-A antibody works.

    Please Let me know what I can try next or if I should try a different GABA A antibody or a positive control?

    Please advise!

    Read More

    Abcam community

    Verified customer

    Asked on Jul 26 2012

    Answer

    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    You are correct in thinking the band expected for the GABA A Receptor alpha 1 would be around 52 kDa. Unfortunately, we have not tested this antibody with guinea pig samples as yet and cannot therefore say conclusively that it will be able to cross react. I would therefore say that using a positive control would be very worthwhile. However, I don't think the HeLa or HepG2 would be suitable as this is a protein mainly expressed in the brain.

    Do you have any access to human, rat or mouse brain lysates? If you do, I would suggest trying these.

    We do not currently have any anti-GABA A Receptor alpha 1 antibodies which have been shown to detect the guinea pig protein but I can checkwhich antibodies may be the most appropriate to try if you would like.


    I look forward to receiving your reply.



    Thank y

    Read More

    Abcam Scientific Support

    Answered on Jul 26 2012

    Question

    Thank you so much for your response. We would like to take advantage of this program and order 3 antibodies. Just to clarify once we submit Abreview with our Flow cytometry data (positive or negative) we will get 3 antibodies for free? Is that correct?   We would be interested in getting the following antibodies:   Anti GABA A receptor alpha 1 subunit antibody: ab33299 (https://www.abcam.com/GABA-A-Receptor-alpha-1-antibody-ab33299.html) Anti GABA A receptor beta 2 subunit antibody: Ab42598 (https://www.abcam.com/GABA-A-Receptor-beta-2-antibody-ab42598.html) Anti GABA B receptor 1 antibody: ab55051 (https://www.abcam.com/GABA-B-Receptor-1-antibody-ab55051.html)   Please let me know if there is a particular quote number we should use while ordering?   Thank you for the information.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 26 2012

    Answer

    I am very pleased to hear you would like to accept our offer and test these products in Flow Cytometry. Each code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for Flow Cytometry for the product and include the related code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. You do not need to referencea quote number when ordering these products. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Apr 26 2012

    Question

    Hello,   We are interested in ordering antibodies for the flow cytometry and I would like to make sure that antibody can get an access to the receptor subunits. Another requirement is that they need to react with human cells.   The antibodies we are interested are the following   Anti GABA A receptor alpha 1 subunit antibody  https://www.abcam.com/GABA-A-Receptor-alpha-1-antibody-ab33299.html Anti GABA A receptor beta 2 subunit antibody  https://www.abcam.com/GABA-A-Receptor-beta-2-antibody-ab42598.html Anti GABA B receptor 1 antibody  https://www.abcam.com/GABA-B-Receptor-1-antibody-ab90883.html   Can you please verify that they will work with flow cytometry before I place an order?   Could you please also provide us a quote for the antibodies?   Thank in advance. We appreciate your help.   Best wishes,

    Read More

    Abcam community

    Verified customer

    Asked on Apr 24 2012

    Answer

    Thank you for contacting us.

    I have had a chance to review each of the product which you have listed as well as our other products against the same targets. I would like to offer some recommendations.

    Anti GABA A receptor alpha 1 subunit antibody: ab33299 (https://www.abcam.com/GABA-A-Receptor-alpha-1-antibody-ab33299.html) and ab86320 (https://www.abcam.com/GABA-A-Receptor-alpha-1-antibody-ab86320.html) will both recognize the extracellular region of this protein in human tissue. Each will work as well as the other with the only difference being that ab86320 was created fromHuman GABA A Receptor alpha 1 andhas 100% homology, while ab33299 is created fromRat GABA A Receptor alpha 1with 93% homology.

    Anti GABA A receptor beta 2 subunit antibody: Ab42598 (https://www.abcam.com/GABA-A-Receptor-beta-2-antibody-ab42598.html)is raised against the extracellular region of this protein and will react with human tissues.

    Anti GABA B receptor 1 antibody: ab90883 (https://www.abcam.com/GABA-B-Receptor-1-antibody-ab90883.html) recognizes a cytoplasmic region of this protein and will require permeabilzation of the cells to be effective. Alternately ab55051 (https://www.abcam.com/GABA-B-Receptor-1-antibody-ab55051.html) recognizes the extracellular region of this protein. This will also work with human tissues.

    None of our antibodies against these targets have been tested in flow cytometry. As such I may offer you enrollment in our 100% Abreview Testing Discount. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    I hope this information is helpful to you. Please let me know if you have any questions or would like to take advantage of our testing discount with these products.


    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Apr 24 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-GABA A Receptor alpha 1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Retina)
    Specification
    Retina
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 12 2011

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