Anti-GABA A Receptor alpha 1 antibody (ab33299)

Merci de nous avoir contactés.
Voici les liens vers les guides et posters qui je l'espère vous seront utiles:
> DyLight® conjugated secondary antibodies : https://docs.abcam.com/pdf/general/DyLight_flyer.pdf
> A Guide to Fluorochromes : https://docs.abcam.com/pdf/immunology/fluorochrome_guide.pdf
> How to choose the right secondary antibody : https://www.abcam.com/index.html?pageconfig=resource&rid=12825
> How to choose a conjugate : https://www.abcam.com/index.html?pageconfig=resource&rid=12922
J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

You are correct in thinking the band expected for the GABA A Receptor alpha 1 would be around 52 kDa. Unfortunately, we have not tested this antibody with guinea pig samples as yet and cannot therefore say conclusively that it will be able to cross react. I would therefore say that using a positive control would be very worthwhile. However, I don't think the HeLa or HepG2 would be suitable as this is a protein mainly expressed in the brain.

Do you have any access to human, rat or mouse brain lysates? If you do, I would suggest trying these.

We do not currently have any anti-GABA A Receptor alpha 1 antibodies which have been shown to detect the guinea pig protein but I can checkwhich antibodies may be the most appropriate to try if you would like.


I look forward to receiving your reply.



Thank y

I am very pleased to hear you would like to accept our offer and test these products in Flow Cytometry. Each code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for Flow Cytometry for the product and include the related code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. You do not need to referencea quote number when ordering these products. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thank you for contacting us.

I have had a chance to review each of the product which you have listed as well as our other products against the same targets. I would like to offer some recommendations.

Anti GABA A receptor alpha 1 subunit antibody: ab33299 (https://www.abcam.com/GABA-A-Receptor-alpha-1-antibody-ab33299.html) and ab86320 (https://www.abcam.com/GABA-A-Receptor-alpha-1-antibody-ab86320.html) will both recognize the extracellular region of this protein in human tissue. Each will work as well as the other with the only difference being that ab86320 was created fromHuman GABA A Receptor alpha 1 andhas 100% homology, while ab33299 is created fromRat GABA A Receptor alpha 1with 93% homology.

Anti GABA A receptor beta 2 subunit antibody: Ab42598 (https://www.abcam.com/GABA-A-Receptor-beta-2-antibody-ab42598.html)is raised against the extracellular region of this protein and will react with human tissues.

Anti GABA B receptor 1 antibody: ab90883 (https://www.abcam.com/GABA-B-Receptor-1-antibody-ab90883.html) recognizes a cytoplasmic region of this protein and will require permeabilzation of the cells to be effective. Alternately ab55051 (https://www.abcam.com/GABA-B-Receptor-1-antibody-ab55051.html) recognizes the extracellular region of this protein. This will also work with human tissues.

None of our antibodies against these targets have been tested in flow cytometry. As such I may offer you enrollment in our 100% Abreview Testing Discount. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

I hope this information is helpful to you. Please let me know if you have any questions or would like to take advantage of our testing discount with these products.


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