Overview

  • Product name
    Anti-GABA A Receptor alpha 1 antibody
    See all GABA A Receptor alpha 1 primary antibodies
  • Description
    Rabbit polyclonal to GABA A Receptor alpha 1
  • Host species
    Rabbit
  • Specificity
    This antibody is specific for the GABA A Receptor a1 subunit.
  • Tested applications
    Suitable for: IHC-P, WB, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
    Predicted to work with: Cow, Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    QPSQDELKDNTTVFT-C

    , corresponding to N terminal amino acids 1-15 of the mature Rat GABA A Receptor alpha 1 containing a cysteine at the C terminus for linkage to a carrier protein.

  • Positive control
    • WB and IP: Rat brain microsomal tissue extract

Properties

Applications

Our Abpromise guarantee covers the use of ab33299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. PubMed: 20650017
WB 1/10000. Predicted molecular weight: 52 kDa.
IP Use at an assay dependent concentration. 5ul immunoprecipitates the GABA A Receptor a1 subunit in rat brain microsomal preparation RIPA lysate.
IHC-Fr Use at an assay dependent concentration. Note: use 4% para glutaraldehyde fixed frozen sections permeabilized with Triton X100.

Target

  • Function
    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
  • Involvement in disease
    Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
    Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue.
  • Sequence similarities
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.
  • Cellular localization
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ECA4 antibody
    • EIEE19 antibody
    • EJM antibody
    • EJM5 antibody
    • Gaba receptor alpha 1 polypeptide antibody
    • GABA(A) receptor antibody
    • GABA(A) receptor subunit alpha 1 antibody
    • GABA(A) receptor subunit alpha-1 antibody
    • GABA(A) receptor, alpha 1 antibody
    • GABRA 1 antibody
    • GABRA1 antibody
    • Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody
    • Gamma aminobutyric acid A receptor alpha 1 antibody
    • Gamma aminobutyric acid receptor subunit alpha 1 antibody
    • Gamma aminobutyric acid type A receptor alpha1 subunit antibody
    • Gamma-aminobutyric acid receptor subunit alpha-1 antibody
    • GBRA1_HUMAN antibody
    see all

Images

  • Anti-GABA A Receptor alpha 1 antibody (ab33299) at 1/10000 dilution + rat brain microsomal preparation tissue lysate at 10 µg

    Predicted band size: 52 kDa
    Observed band size: 51 kDa
    why is the actual band size different from the predicted?



    Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.

  • Immunohistochemistry analysis of formalin fixed paraffin embedded rat hippocampus tissue prepared using heat-induced epitope retrieval (HIER) labeling GABA A Receptor alpha 1 using ab33299 at 1/500 dilution. Secondary antibody was an anti-rabbit antibody and HRP-DAB.

  • Immunoprecipitation analysis: 1 mg of RIPA lysate of rat brain microsomes
    was immunoprecipitated with 5 uL of ab33299. Arrow indicates GABAA
    Receptor a1 (~50kD).

  • ab33299 staining GABA A Receptor alpha 1 in human retinal tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed with 4% paraformaldehyde, blocked with 10% donkey serum for 20 minutes at 24°C, then incubated with ab33299 at a 1/100 dilution for 16 hours at 4°C. The secondary antibody used was an Alexa-Fluor 488 (green) conjugated donkey anti-rabbit polyclonal, used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).

    IPL - inner plexiform layer
    INL - inner nuclear layer
    OPL - outer plexiform layer
    ONL - outer nuclear layer
    INS - inner nuclear segments

    See Abreview

References

This product has been referenced in:
  • Tabouy L  et al. Dysbiosis of microbiome and probiotic treatment in a genetic model of autism spectrum disorders. Brain Behav Immun N/A:N/A (2018). Read more (PubMed: 29787855) »
  • Aoki N  et al. GABA-A and GABA-B Receptors in Filial Imprinting Linked With Opening and Closing of the Sensitive Period in Domestic Chicks (Gallus gallus domesticus). Front Physiol 9:1837 (2018). Read more (PubMed: 30618842) »
See all 15 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Cell (SH-SY5Y))
Permeabilization
Yes - Triton X-100 (0'25%)
Specification
Human Cell (SH-SY5Y)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 30 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cortical)
Loading amount
20 µg
Specification
Cortical
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Dr. Martin Broadstock

Verified customer

Submitted Aug 16 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Retina)
Specification
Retina
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Sep 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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